論文 - 詳細
| RRC ID | 53421 |
|---|---|
| 著者 | Monen J, Hattersley N, Muroyama A, Stevens D, Oegema K, Desai A. |
| タイトル | Separase Cleaves the N-Tail of the CENP-A Related Protein CPAR-1 at the Meiosis I Metaphase-Anaphase Transition in C. elegans. |
| ジャーナル | PLoS One |
| Abstract |
Centromeres are defined epigenetically in the majority of eukaryotes by the presence of chromatin containing the centromeric histone H3 variant CENP-A. Most species have a single gene encoding a centromeric histone variant whereas C. elegans has two: HCP-3 (also known as CeCENP-A) and CPAR-1. Prior RNAi replacement experiments showed that HCP-3 is the functionally dominant isoform, consistent with CPAR-1 not being detectable in embryos. GFP::CPAR-1 is loaded onto meiotic chromosomes in diakinesis and is enriched on bivalents until meiosis I. Here we show that GFP::CPAR-1 signal loss from chromosomes precisely coincides with homolog segregation during anaphase I. This loss of GFP::CPAR-1 signal reflects proteolytic cleavage between GFP and the histone fold of CPAR-1, as CPAR-1::GFP, in which GFP is fused to the C-terminus of CPAR-1, does not exhibit any loss of GFP signal. A focused candidate screen implicated separase, the protease that initiates anaphase by cleaving the kleisin subunit of cohesin, in this cleavage reaction. Examination of the N-terminal tail sequence of CPAR-1 revealed a putative separase cleavage site and mutation of the signature residues in this site eliminated the cleavage reaction, as visualized by retention of GFP::CPAR-1 signal on separating homologous chromosomes at the metaphase-anaphase transition of meiosis I. Neither cleaved nor uncleavable CPAR-1 were centromere-localized in mitosis and instead localized throughout chromatin, indicating that centromere activity has not been retained in CPAR-1. Although the functions of CPAR-1 and of its separase-dependent cleavage remain to be elucidated, this effort reveals a new substrate of separase and provides an in vivo biosensor to monitor separase activity at the onset of meiosis I anaphase. |
| 巻・号 | 10(4) |
| ページ | e0125382 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0125382 |
| PII | PONE-D-14-55696 |
| PMID | 25919583 |
| PMC | PMC4412405 |
| MeSH | Amino Acid Sequence Anaphase* Animals Autoantigens / metabolism* Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / metabolism Cell Cycle Proteins / chemistry* Cell Cycle Proteins / metabolism Centromere / metabolism Centromere Protein A Chromosomal Proteins, Non-Histone / metabolism* Chromosome Segregation Chromosomes / metabolism Gene Duplication Green Fluorescent Proteins / metabolism Histones / metabolism Meiosis* Metaphase* Molecular Sequence Data Mutation / genetics Oocytes / cytology Oocytes / metabolism Separase / antagonists & inhibitors Separase / metabolism* |
| IF | 2.74 |
| 引用数 | 10 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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