Reference - Detail
| RRC ID | 53471 |
|---|---|
| Author | Tang W, Tu S, Lee HC, Weng Z, Mello CC. |
| Title | The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans. |
| Journal | Cell |
| Abstract |
Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and are essential for fertility in diverse organisms. An interesting feature of piRNAs is that, while piRNA lengths are stereotypical within a species, they can differ widely between species. For example, piRNAs are mainly 29 and 30 nucleotides in humans, 24 to 30 nucleotides in D. melanogaster, and uniformly 21 nucleotides in C. elegans. However, how piRNA length is determined and whether length impacts function remains unknown. Here, we show that C. elegans deficient for PARN-1, a conserved RNase, accumulate untrimmed piRNAs with 3' extensions. Surprisingly, these longer piRNAs are stable and associate with the Piwi protein PRG-1 but fail to robustly recruit downstream silencing factors. Our findings identify PARN-1 as a key regulator of piRNA length in C. elegans and suggest that length is regulated to promote efficient transcriptome surveillance. |
| Volume | 164(5) |
| Pages | 974-84 |
| Published | 2016-2-25 |
| DOI | 10.1016/j.cell.2016.02.008 |
| PII | S0092-8674(16)30074-5 |
| PMID | 26919432 |
| PMC | PMC4785802 |
| MeSH | Amino Acid Sequence Animals Argonaute Proteins / metabolism Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Exoribonucleases / chemistry Exoribonucleases / metabolism* Metabolic Networks and Pathways Molecular Sequence Data Mutation RNA Processing, Post-Transcriptional* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Sequence Alignment Transcriptome |
| IF | 38.637 |
| Times Cited | 52 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm872 tm1339 tm4477 tm869 |