| RRC ID |
53543
|
| 著者 |
Hirai K, Wang Z, Miura K, Hayashi T, Awasaki T, Wada M, Keira Y, Ishikawa HO, Sawamura K.
|
| タイトル |
Genetic Analyses of Elys Mutations in Drosophila Show Maternal-Effect Lethality and Interactions with Nucleoporin Genes.
|
| ジャーナル |
G3 (Bethesda)
|
| Abstract |
ELYS determines the subcellular localizations of Nucleoporins (Nups) during interphase and mitosis. We made loss-of-function mutations of Elys in Drosophila melanogaster and found that ELYS is dispensable for zygotic viability and male fertility but the maternal supply is necessary for embryonic development. Subsequent to fertilization, mitotic progression of the embryos produced by the mutant females is severely disrupted at the first cleavage division, accompanied by irregular behavior of mitotic centrosomes. The Nup160 introgression from D. simulans shows close resemblance to that of the Elys mutations, suggesting a common role for those proteins in the first cleavage division. Our genetic experiments indicated critical interactions between ELYS and three Nup107-160 subcomplex components; hemizygotes of either Nup37, Nup96 or Nup160 were lethal in the genetic background of the Elys mutation. Not only Nup96 and Nup160 but also Nup37 of D. simulans behave as recessive hybrid incompatibility genes with D. melanogaster An evolutionary analysis indicated positive natural selection in the ELYS-like domain of ELYS. Here we propose that genetic incompatibility between Elys and Nups may lead to reproductive isolation between D. melanogaster and D. simulans, although direct evidence is necessary.
|
| 巻・号 |
8(7)
|
| ページ |
2421-2431
|
| 公開日 |
2018-7-2
|
| DOI |
10.1534/g3.118.200361
|
| PII |
g3.118.200361
|
| PMID |
29773558
|
| PMC |
PMC6027884
|
| MeSH |
Animals
Crosses, Genetic
Drosophila / genetics*
Drosophila Proteins / genetics*
Epistasis, Genetic*
Evolution, Molecular
Female
Genes, Lethal*
Genotype
Loss of Function Mutation
Male
Maternal Inheritance*
Mitosis / genetics
Mutation*
Nuclear Pore Complex Proteins / genetics*
Phenotype
Synthetic Lethal Mutations
|
| IF |
2.781
|
| 引用数 |
1
|
| リソース情報 |
| ショウジョウバエ |
DGRC#118829
DGRC#118830 |
