論文 - 詳細
| RRC ID | 53958 |
|---|---|
| 著者 | Zhang T, Wu YC, Mullane P, Ji YJ, Liu H, He L, Arora A, Hwang HY, Alessi AF, Niaki AG, Periz G, Guo L, Wang H, Elkayam E, Joshua-Tor L, Myong S, Kim JK, Shorter J, Ong SE, Leung AKL, Wang J. |
| タイトル | FUS Regulates Activity of MicroRNA-Mediated Gene Silencing. |
| ジャーナル | Mol Cell |
| Abstract |
MicroRNA-mediated gene silencing is a fundamental mechanism in the regulation of gene expression. It remains unclear how the efficiency of RNA silencing could be influenced by RNA-binding proteins associated with the microRNA-induced silencing complex (miRISC). Here we report that fused in sarcoma (FUS), an RNA-binding protein linked to neurodegenerative diseases including amyotrophic lateral sclerosis (ALS), interacts with the core miRISC component AGO2 and is required for optimal microRNA-mediated gene silencing. FUS promotes gene silencing by binding to microRNA and mRNA targets, as illustrated by its action on miR-200c and its target ZEB1. A truncated mutant form of FUS that leads its carriers to an aggressive form of ALS, R495X, impairs microRNA-mediated gene silencing. The C. elegans homolog fust-1 also shares a conserved role in regulating the microRNA pathway. Collectively, our results suggest a role for FUS in regulating the activity of microRNA-mediated silencing. |
| 巻・号 | 69(5) |
| ページ | 787-801.e8 |
| 公開日 | 2018-3-1 |
| DOI | 10.1016/j.molcel.2018.02.001 |
| PII | S1097-2765(18)30099-6 |
| PMID | 29499134 |
| PMC | PMC5836505 |
| MeSH | Amyotrophic Lateral Sclerosis / genetics Amyotrophic Lateral Sclerosis / metabolism Amyotrophic Lateral Sclerosis / pathology Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Silencing* HEK293 Cells Humans Mice MicroRNAs / genetics MicroRNAs / metabolism* RNA, Helminth / genetics RNA, Helminth / metabolism* RNA-Binding Protein FUS / genetics RNA-Binding Protein FUS / metabolism* |
| IF | 15.584 |
| 引用数 | 17 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 32 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1 tm4439 |