RRC ID 54084
Author Zhong F, Xu J, Yang X, Zhang Q, Gao Z, Deng Y, Zhang L, Yu C.
Title miR-145 eliminates lipopolysaccharides-induced inflammatory injury in human fibroblast-like synoviocyte MH7A cells.
Journal J Cell Biochem
Abstract Recently, it has been accepted that miR-based therapy may be beneficial for rheumatoid arthritis (RA). This study aimed to evaluate the potential involvement of miR-145 in RA in vitro. The expression of miR-145 in the human fibroblast-like synoviocyte line MH7A was overexpressed by miR-mimic transfection, after which cells were subjected to lipopolysaccharides (LPS). Cell viability, apoptosis, and the release of pro-inflammatory cytokines were measured. The result showed that the apoptosis and the release of IL-1β, IL-6, IL-8, and TNF-α were significantly induced by LPS. Meanwhile, LPS treatment led to downregulation of miR-145. miR-145 overexpression in LPS-untreated MH7A cells had no impacts on cell apoptosis and inflammation. But, restoring miR-145 expression in LPS-stimulated cells by supplementation of a miR-145 mimic protected MH7A cells against LPS-induced apoptosis and inflammation. Furthermore, miR-145 overexpression in LPS-untreated MH7A cells slightly blocked the PI3K/ATK and mTOR pathways, whereas miR-145 overexpression in LPS-stimulated cells notably repressed the LPS-induced activation of PI3K/ATK and MAPK/mTOR pathways. Our study suggested that miR-145 protected MH7A cells against LPS-induced apoptosis and inflammation by inhibiting the PI3K/AKT and MAPK/mTOR pathways.
Volume 119(12)
Pages 10059-10066
Published 2018-12-1
DOI 10.1002/jcb.27341
PMID 30191608
MeSH Apoptosis / genetics Arthritis, Rheumatoid / genetics* Arthritis, Rheumatoid / pathology Cell Line Cell Survival / genetics Fibroblasts / metabolism Fibroblasts / pathology Gene Expression Regulation / genetics Humans Inflammation / chemically induced Inflammation / genetics* Inflammation / pathology Lipopolysaccharides / toxicity MAP Kinase Kinase 1 / genetics MicroRNAs / genetics* Phosphatidylinositol 3-Kinases / genetics Proto-Oncogene Proteins c-akt / genetics Signal Transduction / genetics Synovial Fluid / metabolism Synoviocytes / metabolism* Synoviocytes / pathology TOR Serine-Threonine Kinases / genetics Tumor Necrosis Factor-alpha / genetics
IF 3.448
Times Cited 3
Human and Animal Cells MH7A(RCB1512)