論文 - 詳細
| RRC ID | 54188 |
|---|---|
| 著者 | Mioka T, Fujimura-Kamada K, Mizugaki N, Kishimoto T, Sano T, Nunome H, Williams DE, Andersen RJ, Tanaka K. |
| タイトル | Phospholipid flippases and Sfk1p, a novel regulator of phospholipid asymmetry, contribute to low permeability of the plasma membrane. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Phospholipid flippase (type 4 P-type ATPase) plays a major role in the generation of phospholipid asymmetry in eukaryotic cell membranes. Loss of Lem3p-Dnf1/2p flippases leads to the exposure of phosphatidylserine (PS) and phosphatidylethanolamine (PE) on the cell surface in yeast, resulting in sensitivity to PS- or PE-binding peptides. We isolated Sfk1p, a conserved membrane protein in the TMEM150/FRAG1/DRAM family, as a multicopy suppressor of this sensitivity. Overexpression of SFK1 decreased PS/PE exposure in lem3Δ mutant cells. Consistent with this, lem3Δ sfk1Δ double mutant cells exposed more PS/PE than the lem3Δ mutant. Sfk1p was previously implicated in the regulation of the phosphatidylinositol-4 kinase Stt4p, but the effect of Sfk1p on PS/PE exposure in lem3Δ was independent of Stt4p. Surprisingly, Sfk1p did not facilitate phospholipid flipping but instead repressed it, even under ATP-depleted conditions. We propose that Sfk1p negatively regulates transbilayer movement of phospholipids irrespective of directions. In addition, we showed that the permeability of the plasma membrane was dramatically elevated in the lem3Δ sfk1Δ double mutant in comparison with the corresponding single mutants. Interestingly, total ergosterol was decreased in the lem3Δ sfk1Δ mutant. Our results suggest that phospholipid asymmetry is required for the maintenance of low plasma membrane permeability. |
| 巻・号 | 29(10) |
| ページ | 1203-1218 |
| 公開日 | 2018-5-15 |
| DOI | 10.1091/mbc.E17-04-0217 |
| PII | mbc.E17-04-0217 |
| PMID | 29540528 |
| PMC | PMC5935070 |
| MeSH | Amino Acid Sequence Cell Membrane / metabolism* Cell Membrane Permeability* Depsipeptides / pharmacology Ergosterol / pharmacology Lipid Bilayers / metabolism Membrane Fluidity / drug effects Membrane Proteins / chemistry Membrane Proteins / metabolism* Models, Biological Mutation / genetics Phosphatidylethanolamines / metabolism Phosphatidylinositols / metabolism Phosphatidylserines / metabolism Phospholipid Transfer Proteins / chemistry Phospholipid Transfer Proteins / metabolism* Phospholipids / metabolism* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / metabolism* Stress, Physiological / drug effects Up-Regulation / drug effects |
| IF | 3.791 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | YEp24-SFK1 (RDB16713) YEplac195-SF1 (RDB16714) YEplac195-SFK1 delta C (RDB16715) |