RRC ID 5605
Author Reimer MM, Sörensen I, Kuscha V, Frank RE, Liu C, Becker CG, Becker T.
Title Motor neuron regeneration in adult zebrafish.
Journal J. Neurosci.
Abstract The mammalian spinal cord does not regenerate motor neurons that are lost as a result of injury or disease. Here we demonstrate that adult zebrafish, which show functional spinal cord regeneration, are capable of motor neuron regeneration. After a spinal lesion, the ventricular zone shows a widespread increase in proliferation, including slowly proliferating olig2-positive (olig2+) ependymo-radial glial progenitor cells. Lineage tracing in olig2:green fluorescent protein transgenic fish indicates that these cells switch from a gliogenic phenotype to motor neuron production. Numbers of undifferentiated small HB9+ and islet-1+ motor neurons, which are double labeled with the proliferation marker 5-bromo-2-deoxyuridine (BrdU), are transiently strongly increased in the lesioned spinal cord. Large differentiated motor neurons, which are lost after a lesion, reappear at 6-8 weeks after lesion, and we detected ChAT+/BrdU+ motor neurons that were covered by contacts immunopositive for the synaptic marker SV2. These observations suggest that, after a lesion, plasticity of olig2+ progenitor cells may allow them to generate motor neurons, some of which exhibit markers for terminal differentiation and integration into the existing adult spinal circuitry.
Volume 28(34)
Pages 8510-6
Published 2008-8-20
DOI 10.1523/JNEUROSCI.1189-08.2008
PII 28/34/8510
PMID 18716209
MeSH Animals Animals, Genetically Modified Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Bromodeoxyuridine Cell Count Cell Differentiation Cell Lineage Cell Proliferation Green Fluorescent Proteins / genetics Homeodomain Proteins / genetics Homeodomain Proteins / metabolism LIM-Homeodomain Proteins Microscopy, Electron Motor Neurons* / pathology Nerve Regeneration* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neuroglia / metabolism Neuroglia / pathology Oligodendrocyte Transcription Factor 2 Phenotype Recombinant Fusion Proteins / genetics Spinal Cord / metabolism Spinal Cord / pathology Spinal Cord Injuries / pathology Spinal Cord Injuries / physiopathology* Stem Cells / metabolism Stem Cells / pathology Transcription Factors / genetics Transcription Factors / metabolism Zebrafish* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 5.971
Times Cited 110
WOS Category NEUROSCIENCES
Resource
Zebrafish Tg(isl1:GFP)rw0