Reference - Detail
| RRC ID | 56167 |
|---|---|
| Author | Herrera LA, Starr DA. |
| Title | The E3 Ubiquitin Ligase MIB-1 Is Necessary To Form the Nuclear Halo in Caenorhabditis elegans Sperm. |
| Journal | G3 (Bethesda) |
| Abstract |
Unlike the classical nuclear envelope with two membranes found in other eukaryotic cells, most nematode sperm nuclei are not encapsulated by membranes. Instead, they are surrounded by a nuclear halo of unknown composition. How the halo is formed and regulated is unknown. We used forward genetics to identify molecular lesions behind three classical fer (fertilization defective) mutations that disrupt the ultrastructure of the Caenorhabditis elegans sperm nuclear halo. We found fer-2 and fer-4 alleles to be nonsense mutations in mib-1. fer-3 was caused by a nonsense mutation in eri-3 GFP::MIB-1 was expressed in the germline during early spermatogenesis, but not in mature sperm. mib-1 encodes a conserved E3 ubiquitin ligase homologous to vertebrate Mib1 and Mib2, which function in Notch signaling. Here, we show that mib-1 is important for male sterility and is involved in the regulation or formation of the nuclear halo during nematode spermatogenesis. |
| Volume | 8(7) |
| Pages | 2465-2470 |
| Published | 2018-7-2 |
| DOI | 10.1534/g3.118.200426 |
| PII | g3.118.200426 |
| PMID | 29776970 |
| PMC | PMC6027868 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Cell Nucleus / genetics* DNA Mutational Analysis Male Mutation Protein Transport Spermatogenesis / genetics Spermatozoa / cytology* Spermatozoa / metabolism* Ubiquitin-Protein Ligases / genetics* Ubiquitin-Protein Ligases / metabolism |
| IF | 2.63 |
| Times Cited | 0 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1361 |