Reference - Detail
| RRC ID | 5683 |
|---|---|
| Author | Pant S, Sharma M, Patel K, Caplan S, Carr CM, Grant BD. |
| Title | AMPH-1/Amphiphysin/Bin1 functions with RME-1/Ehd1 in endocytic recycling. |
| Journal | Nat Cell Biol |
| Abstract |
RME-1/EHD1 (receptor mediated endocytosis/Eps15 homology-domain containing 1) family proteins are key residents of the recycling endosome, which are required for endosome-to-plasma membrane transport in Caenorhabditis elegans and mammals. Recent studies suggest similarities between the RME-1/EHD proteins and the Dynamin GTPase superfamily of mechanochemical pinchases, which promote membrane fission. Here we show that endogenous C. elegans AMPH-1, the only C. elegans member of the Amphiphysin/BIN1 family of BAR (Bin1-Amphiphysin-Rvs161p/167p)-domain-containing proteins, colocalizes with RME-1 on recycling endosomes in vivo, that amph-1-deletion mutants are defective in recycling endosome morphology and function, and that binding of AMPH-1 Asn-Pro-Phe(Asp/Glu) sequences to the RME-1 EH-domain promotes the recycling of transmembrane cargo. We also show a requirement for human BIN1 (also known as Amphiphysin 2) in EHD1-regulated endocytic recycling. In vitro, we find that purified recombinant AMPH-1-RME-1 complexes produce short, coated membrane tubules that are qualitatively distinct from those produced by either protein alone. Our results indicate that AMPH-1 and RME-1 cooperatively regulate endocytic recycling, probably through functions required for the production of cargo carriers that exit the recycling endosome for the cell surface. |
| Volume | 11(12) |
| Pages | 1399-410 |
| Published | 2009-12-1 |
| DOI | 10.1038/ncb1986 |
| PII | ncb1986 |
| PMID | 19915558 |
| PMC | PMC2788952 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / ultrastructure Endocytosis* HeLa Cells Humans Microscopy, Electron Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Nerve Tissue Proteins / ultrastructure Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding RNA Interference Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism* |
| IF | 20.042 |
| Times Cited | 120 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1060 |