Reference - Detail
| RRC ID | 5684 |
|---|---|
| Author | Shi A, Sun L, Banerjee R, Tobin M, Zhang Y, Grant BD. |
| Title | Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8. |
| Journal | EMBO J |
| Abstract |
After endocytosis, most cargo enters the pleiomorphic early endosomes in which sorting occurs. As endosomes mature, transmembrane cargo can be sequestered into inwardly budding vesicles for degradation, or can exit the endosome in membrane tubules for recycling to the plasma membrane, the recycling endosome, or the Golgi apparatus. Endosome to Golgi transport requires the retromer complex. Without retromer, recycling cargo such as the MIG-14/Wntless protein aberrantly enters the degradative pathway and is depleted from the Golgi. Endosome-associated clathrin also affects the recycling of retrograde cargo and has been shown to function in the formation of endosomal subdomains. Here, we find that the Caemorhabditis elegans endosomal J-domain protein RME-8 associates with the retromer component SNX-1. Loss of SNX-1, RME-8, or the clathrin chaperone Hsc70/HSP-1 leads to over-accumulation of endosomal clathrin, reduced clathrin dynamics, and missorting of MIG-14 to the lysosome. Our results indicate a mechanism, whereby retromer can regulate endosomal clathrin dynamics through RME-8 and Hsc70, promoting the sorting of recycling cargo into the retrograde pathway. |
| Volume | 28(21) |
| Pages | 3290-302 |
| Published | 2009-11-4 |
| DOI | 10.1038/emboj.2009.272 |
| PII | emboj2009272 |
| PMID | 19763082 |
| PMC | PMC2776105 |
| MeSH | Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / analysis Carrier Proteins / metabolism Cell Line Cell Polarity Clathrin / metabolism* Endosomes / metabolism* Golgi Apparatus / genetics HSC70 Heat-Shock Proteins / metabolism Intracellular Signaling Peptides and Proteins Lysosomes / metabolism Molecular Chaperones Mutation Neurons / cytology Sorting Nexins Vesicular Transport Proteins / analysis Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 9.889 |
| Times Cited | 94 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| C.elegans | snx-1(tm0847) tm847 tm857 |