RRC ID 5694
Author Satoh D, Sato D, Tsuyama T, Saito M, Ohkura H, Rolls MM, Ishikawa F, Uemura T.
Title Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes.
Journal Nat Cell Biol
Abstract Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs. Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. This phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.
Volume 10(10)
Pages 1164-71
Published 2008-10-1
DOI 10.1038/ncb1776
PII ncb1776
PMID 18758452
MeSH Animals Axons / metabolism Biological Transport Carrier Proteins / metabolism Clone Cells Dendrites / metabolism* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Dyneins / metabolism* Endosomes / metabolism* Genes, Insect Kinesin / metabolism Morphogenesis Mutation / genetics Phenotype rab5 GTP-Binding Proteins / deficiency rab5 GTP-Binding Proteins / metabolism*
IF 17.728
Times Cited 127
WOS Category CELL BIOLOGY
Resource
Drosophila