RRC ID 5696
Author Shimada Y, Yonemura S, Ohkura H, Strutt D, Uemura T.
Title Polarized transport of Frizzled along the planar microtubule arrays in Drosophila wing epithelium.
Journal Dev. Cell
Abstract Cells in a variety of developmental contexts sense extracellular cues that are given locally on their surfaces, and subsequently amplify the initial signal to achieve cell polarization. Drosophila wing cells acquire planar polarity along the proximal-distal (P-D) axis, in which the amplification of the presumptive cue involves assembly of a multiprotein complex that spans distal and proximal boundaries of adjacent cells. Here we pursue the mechanisms that place one of the components, Frizzled (Fz), at the distal side. Intracellular particles of GFP-tagged Fz moved preferentially toward distal boundaries before Fz::GFP and other components were tightly localized at the P/D cortex. Arrays of microtubules (MTs) were approximately oriented along the P-D axis and these MTs contributed to the formation of the cortical complex. Furthermore, there appeared to be a bias in the P-D MTs, with slightly more plus ends oriented distally. The hypothesis of polarized vesicular trafficking of Fz is discussed.
Volume 10(2)
Pages 209-22
Published 2006-2
DOI 10.1016/j.devcel.2005.11.016
PII S1534-5807(05)00476-4
PMID 16459300
MeSH Adaptor Proteins, Signal Transducing Adherens Junctions / metabolism Animals Animals, Genetically Modified Biological Transport, Active Cadherins / metabolism Cell Polarity Dishevelled Proteins Drosophila / cytology* Drosophila / genetics Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Epithelial Cells / cytology Epithelial Cells / metabolism Frizzled Receptors Genes, Insect Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism* Microscopy, Electron Microtubules / metabolism Models, Biological Mutation Phosphoproteins / metabolism Pupa / cytology Pupa / metabolism Receptors, G-Protein-Coupled Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction Wings, Animal / cytology Wings, Animal / metabolism
IF 9.19
Times Cited 162