| RRC ID |
5696
|
| 著者 |
Shimada Y, Yonemura S, Ohkura H, Strutt D, Uemura T.
|
| タイトル |
Polarized transport of Frizzled along the planar microtubule arrays in Drosophila wing epithelium.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Cells in a variety of developmental contexts sense extracellular cues that are given locally on their surfaces, and subsequently amplify the initial signal to achieve cell polarization. Drosophila wing cells acquire planar polarity along the proximal-distal (P-D) axis, in which the amplification of the presumptive cue involves assembly of a multiprotein complex that spans distal and proximal boundaries of adjacent cells. Here we pursue the mechanisms that place one of the components, Frizzled (Fz), at the distal side. Intracellular particles of GFP-tagged Fz moved preferentially toward distal boundaries before Fz::GFP and other components were tightly localized at the P/D cortex. Arrays of microtubules (MTs) were approximately oriented along the P-D axis and these MTs contributed to the formation of the cortical complex. Furthermore, there appeared to be a bias in the P-D MTs, with slightly more plus ends oriented distally. The hypothesis of polarized vesicular trafficking of Fz is discussed.
|
| 巻・号 |
10(2)
|
| ページ |
209-22
|
| 公開日 |
2006-2-1
|
| DOI |
10.1016/j.devcel.2005.11.016
|
| PII |
S1534-5807(05)00476-4
|
| PMID |
16459300
|
| MeSH |
Adaptor Proteins, Signal Transducing
Adherens Junctions / metabolism
Animals
Animals, Genetically Modified
Biological Transport, Active
Cadherins / metabolism
Cell Polarity
Dishevelled Proteins
Drosophila / cytology*
Drosophila / genetics
Drosophila / metabolism*
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Epithelial Cells / cytology
Epithelial Cells / metabolism
Frizzled Receptors
Genes, Insect
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Microscopy, Electron
Microtubules / metabolism
Models, Biological
Mutation
Phosphoproteins / metabolism
Pupa / cytology
Pupa / metabolism
Receptors, G-Protein-Coupled
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Signal Transduction
Wings, Animal / cytology
Wings, Animal / metabolism
|
| IF |
10.092
|
| 引用数 |
190
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
|
