論文 - 詳細
| RRC ID | 57096 |
|---|---|
| 著者 | Homma Y, Kinoshita R, Kuchitsu Y, Wawro PS, Marubashi S, Oguchi ME, Ishida M, Fujita N, Fukuda M. |
| タイトル | Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. |
| ジャーナル | J Cell Biol |
| Abstract |
The Rab family of small GTPases comprises the largest number of proteins (∼60 in mammals) among the regulators of intracellular membrane trafficking, but the precise function of many Rabs and the functional redundancy and diversity of Rabs remain largely unknown. Here, we generated a comprehensive collection of knockout (KO) MDCK cells for the entire Rab family. We knocked out closely related paralogs simultaneously (Rab subfamily knockout) to circumvent functional compensation and found that Rab1A/B and Rab5A/B/C are critical for cell survival and/or growth. In addition, we demonstrated that Rab6-KO cells lack the basement membrane, likely because of the inability to secrete extracellular matrix components. Further analysis revealed the general requirement of Rab6 for secretion of soluble cargos. Transport of transmembrane cargos to the plasma membrane was also significantly delayed in Rab6-KO cells, but the phenotype was relatively mild. Our Rab-KO collection, which shares the same background, would be a valuable resource for analyzing a variety of membrane trafficking events. |
| 巻・号 | 218(6) |
| ページ | 2035-2050 |
| 公開日 | 2019-6-3 |
| DOI | 10.1083/jcb.201810134 |
| PII | jcb.201810134 |
| PMID | 31072826 |
| PMC | PMC6548125 |
| MeSH | Animals Cell Membrane / metabolism* Dogs Epithelial Cells / cytology* Epithelial Cells / metabolism Gene Knockout Techniques / methods Guanosine Triphosphate / metabolism* HEK293 Cells Humans Intracellular Membranes Madin Darby Canine Kidney Cells Multiprotein Complexes / metabolism* Organelles / physiology* Phenotype Protein Transport RNA, Small Interfering / genetics* rab GTP-Binding Proteins / antagonists & inhibitors* rab GTP-Binding Proteins / genetics rab GTP-Binding Proteins / metabolism |
| IF | 8.891 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 101 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pMRX-bsr-Myc-Rab2A (RDB17357) pMRX-bsr-Myc-Rab5A (RDB17359) pMRX-puro-EGFP-Rab6A (RDB17361) pMRX-puro-EGFP-Rab11A (RDB17362) pMRX-bsr-Myc-Rab2B (RDB17358) pMRX-puro-EGFP-Rab1B (RDB17360) pMRX-bsr-ss-EGFP-FM4 (RDB17364) pMRX-bsr-ss-EGFP-FM4-TMD (RDB17365) pMRX-bsr-ss-EGFP (RDB17363) |