論文 - 詳細
| RRC ID | 57115 |
|---|---|
| 著者 | Fujisawa Y, Kosakamoto H, Chihara T, Miura M. |
| タイトル | Non-apoptotic function of Drosophila caspase activation in epithelial thorax closure and wound healing. |
| ジャーナル | Development |
| Abstract |
Non-apoptotic caspase activation involves multiple cellular events. However, the link between visible non-apoptotic caspase activation and its function in living organisms has not yet been revealed. Here, we visualized sub-lethal activation of apoptotic signaling with the combination of a sensitive indicator for caspase 3 activation and in vivo live-imaging analysis of Drosophila During thorax closure in pupal development, caspase 3 activation was specifically observed at the leading edge cells, with no signs of apoptosis. Inhibition of caspase activation led to an increase in thorax closing speed, which suggests a role of non-apoptotic caspase activity in cell motility. Importantly, sub-lethal activation of caspase 3 was also observed during wound closure at the fusion sites at which thorax closure had previously taken place. Further genetic analysis revealed that the activation of the initiator caspase Dronc is coupled with the generation of reactive oxygen species. The activation of Dronc also regulates myosin levels and delays wound healing. Our findings suggest a possible function for non-apoptotic caspase activation in the fine-tuning of cell migratory behavior during epithelial closure. |
| 巻・号 | 146(4) |
| 公開日 | 2019-2-15 |
| DOI | 10.1242/dev.169037 |
| PII | 146/4/dev169037 |
| PMID | 30770378 |
| MeSH | Animals Apoptosis* Caspases / metabolism* Cell Movement Crosses, Genetic Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Enzyme Activation Epithelium / embryology* Epithelium / metabolism Gene Expression Regulation, Developmental Green Fluorescent Proteins / metabolism MAP Kinase Kinase 4 / metabolism Protein Binding Protein Processing, Post-Translational Reactive Oxygen Species / metabolism Signal Transduction Thorax / embryology* Thorax / metabolism Wound Healing* |
| IF | 5.763 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#109007 DGRC#108018 |