RRC ID 57171
著者 Yamakawa T, Atsumi Y, Kubo S, Yamagishi A, Morita I, Matsuno K.
タイトル Insight into Notch Signaling Steps That Involve pecanex from Dominant-Modifier Screens in Drosophila.
ジャーナル Genetics
Abstract Notch signaling plays crucial roles in intercellular communications. In Drosophila, the pecanex (pcx) gene, which encodes an evolutionarily conserved multi-pass transmembrane protein, appears to be required to activate Notch signaling in some contexts, especially during neuroblast segregation in the neuroectoderm. Although Pcx has been suggested to contribute to endoplasmic reticulum homeostasis, its functions remain unknown. Here, to elucidate these roles, we performed genetic modifier screens of pcx We found that pcx heterozygotes lacking its maternal contribution exhibit cold-sensitive lethality, which is attributed to a reduction in Notch signaling at decreased temperatures. Using sets of deletions that uncover most of the second and third chromosomes, we identified four enhancers and two suppressors of the pcx cold-sensitive lethality. Among these, five genes encode known Notch-signaling components: big brain, Delta (Dl), neuralized (neur), Brother of Bearded A (BobA), a member of the Bearded (Brd) family, and N-ethylmaleimide-sensitive factor 2 (Nsf2). We showed that BobA suppresses Dl endocytosis during neuroblast segregation in the neuroectoderm, as Brd family genes reportedly do in the mesoderm for mesectoderm specification. Analyses of Nsf2, a key regulator of vesicular fusion, suggested a novel role in neuroblast segregation, which is distinct from Nsf2's previously reported role in imaginal tissues. Finally, jim lovell, which encodes a potential transcription factor, may play a role in Notch signaling during neuroblast segregation. These results reveal new research avenues for Pcx functions and Notch signaling.
巻・号 209(4)
ページ 1099-1119
公開日 2018-8-1
DOI 10.1534/genetics.118.300935
PII genetics.118.300935
PMID 29853475
PMC PMC6063225
MeSH Animals Cold Temperature DNA-Binding Proteins / metabolism Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Female Gene Expression Regulation, Developmental Genes, Lethal Intracellular Signaling Peptides and Proteins / metabolism Membrane Proteins / genetics* Membrane Proteins / metabolism* N-Ethylmaleimide-Sensitive Proteins / metabolism Receptors, Notch / metabolism Signal Transduction* Stress, Physiological Ubiquitin-Protein Ligases / metabolism
IF 3.564
引用数 0
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