論文 - 詳細
| RRC ID | 57191 |
|---|---|
| 著者 | Zong W, Wang Y, Tang Q, Zhang H, Yu F. |
| タイトル | Prd1 associates with the clathrin adaptor α-Adaptin and the kinesin-3 Imac/Unc-104 to govern dendrite pruning in Drosophila. |
| ジャーナル | PLoS Biol |
| Abstract |
Refinement of the nervous system depends on selective removal of excessive axons/dendrites, a process known as pruning. Drosophila ddaC sensory neurons prune their larval dendrites via endo-lysosomal degradation of the L1-type cell adhesion molecule (L1-CAM), Neuroglian (Nrg). Here, we have identified a novel gene, pruning defect 1 (prd1), which governs dendrite pruning of ddaC neurons. We show that Prd1 colocalizes with the clathrin adaptor protein α-Adaptin (α-Ada) and the kinesin-3 immaculate connections (Imac)/Uncoordinated-104 (Unc-104) in dendrites. Moreover, Prd1 physically associates with α-Ada and Imac, which are both critical for dendrite pruning. Prd1, α-Ada, and Imac promote dendrite pruning via the regulation of endo-lysosomal degradation of Nrg. Importantly, genetic interactions among prd1, α-adaptin, and imac indicate that they act in the same pathway to promote dendrite pruning. Our findings indicate that Prd1, α-Ada, and Imac act together to regulate discrete distribution of α-Ada/clathrin puncta, facilitate endo-lysosomal degradation, and thereby promote dendrite pruning in sensory neurons. |
| 巻・号 | 16(8) |
| ページ | e2004506 |
| 公開日 | 2018-8-1 |
| DOI | 10.1371/journal.pbio.2004506 |
| PII | pbio.2004506 |
| PMID | 30142146 |
| PMC | PMC6126864 |
| MeSH | Adaptor Protein Complex alpha Subunits / genetics* Adaptor Protein Complex alpha Subunits / metabolism Animals Brain / cytology Brain / metabolism Cell Adhesion Molecules, Neuronal / genetics* Cell Adhesion Molecules, Neuronal / metabolism Dendrites / metabolism* Dendrites / ultrastructure Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Endosomes / metabolism Endosomes / ultrastructure Gene Expression Regulation, Developmental Kinesins / genetics* Kinesins / metabolism Larva / genetics Larva / growth & development Larva / metabolism Lysosomes / metabolism Lysosomes / ultrastructure Metamorphosis, Biological / genetics Neural Cell Adhesion Molecule L1 / genetics* Neural Cell Adhesion Molecule L1 / metabolism Neuronal Plasticity / genetics* Protein Binding Proteolysis Sensory Receptor Cells / cytology Sensory Receptor Cells / metabolism Signal Transduction |
| IF | 8.386 |
| 引用数 | 8 |
| リソース情報 | |
| ショウジョウバエ | |