論文 - 詳細
| RRC ID | 57197 |
|---|---|
| 著者 | Walther RF, Burki M, Pinal N, Rogerson C, Pichaud F. |
| タイトル | Rap1, Canoe and Mbt cooperate with Bazooka to promote zonula adherens assembly in the fly photoreceptor. |
| ジャーナル | J Cell Sci |
| Abstract |
In Drosophila epithelial cells, apical exclusion of Bazooka (the Drosophila Par3 protein) defines the position of the zonula adherens (ZA), which demarcates the apical and lateral membrane and allows cells to assemble into sheets. Here, we show that the small GTPase Rap1, its effector Canoe (Cno) and the Cdc42 effector kinase Mushroom bodies tiny (Mbt), converge in regulating epithelial morphogenesis by coupling stabilization of the adherens junction (AJ) protein E-Cadherin and Bazooka retention at the ZA. Furthermore, our results show that the localization of Rap1, Cno and Mbt at the ZA is interdependent, indicating that their functions during ZA morphogenesis are interlinked. In this context, we find the Rap1-GEF Dizzy is enriched at the ZA and our results suggest that it promotes Rap1 activity during ZA morphogenesis. Altogether, we propose the Dizzy, Rap1 and Cno pathway and Mbt converge in regulating the interface between Bazooka and AJ material to promote ZA morphogenesis. |
| 巻・号 | 131(6) |
| 公開日 | 2018-3-26 |
| DOI | 10.1242/jcs.207779 |
| PII | jcs.207779 |
| PMID | 29507112 |
| PMC | PMC5897711 |
| MeSH | Adherens Junctions / genetics Adherens Junctions / metabolism* Animals Cadherins / genetics Cadherins / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Photoreceptor Cells / metabolism* Protein Binding Protein Kinases / genetics Protein Kinases / metabolism* Shelterin Complex Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism* |
| IF | 4.517 |
| 引用数 | 4 |
| リソース情報 | |
| ショウジョウバエ | |