論文 - 詳細
| RRC ID | 57323 |
|---|---|
| 著者 | Hashimoto M, Sasaki H. |
| タイトル | Epiblast Formation by TEAD-YAP-Dependent Expression of Pluripotency Factors and Competitive Elimination of Unspecified Cells. |
| ジャーナル | Dev Cell |
| Abstract |
The epiblast is a pluripotent cell population first formed in preimplantation embryos, and its quality is important for proper development. Here, we examined the mechanisms of epiblast formation and found that the Hippo pathway transcription factor TEAD and its coactivator YAP regulate expression of pluripotency factors. After specification of the inner cell mass, YAP accumulates in the nuclei and activates TEAD. TEAD activity is required for strong expression of pluripotency factors and is variable in the forming epiblast. Cells showing low TEAD activity are eliminated from the epiblast through cell competition. Pluripotency factor expression and MYC control cell competition downstream of TEAD activity. Cell competition eliminates unspecified cells and is required for proper organization of the epiblast. These results suggest that induction of pluripotency factors by TEAD activity and elimination of unspecified cells via cell competition ensure the production of an epiblast with naive pluripotency. |
| 巻・号 | 50(2) |
| ページ | 139-154.e5 |
| 公開日 | 2019-7-22 |
| DOI | 10.1016/j.devcel.2019.05.024 |
| PII | S1534-5807(19)30422-8 |
| PMID | 31204175 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Blastocyst / cytology* Blastocyst / metabolism Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Differentiation* Cell Proliferation Cells, Cultured DNA-Binding Proteins / physiology* Female Germ Layers / cytology* Germ Layers / metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Mouse Embryonic Stem Cells / cytology* Mouse Embryonic Stem Cells / metabolism Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism TEA Domain Transcription Factors Transcription Factors / physiology* YAP-Signaling Proteins |
| IF | 9.19 |
| 引用数 | 12 |
| リソース情報 | |
| 実験動物マウス | RBRC04874 |