RRC ID 57352
Author Khanal I, Elbediwy A, Diaz de la Loza Mdel C, Fletcher GC, Thompson BJ.
Title Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia.
Journal J Cell Sci
Abstract In epithelial tissues, polarisation of microtubules and actin microvilli occurs along the apical-basal axis of each cell, yet how these cytoskeletal polarisation events are coordinated remains unclear. Here, we examine the hierarchy of events during cytoskeletal polarisation in Drosophila melanogaster epithelia. Core apical-basal polarity determinants polarise the spectrin cytoskeleton to recruit the microtubule-binding proteins Patronin (CAMSAP1, CAMSAP2 and CAMSAP3 in humans) and Shortstop [Shot; MACF1 and BPAG1 (also known as DST) in humans] to the apical membrane domain. Patronin and Shot then act to polarise microtubules along the apical-basal axis to enable apical transport of Rab11 endosomes by the Nuf-Dynein microtubule motor complex. Finally, Rab11 endosomes are transferred to the MyoV (also known as Didum in Drosophila) actin motor to deliver the key microvillar determinant Cadherin 99C to the apical membrane to organise the biogenesis of actin microvilli.
Volume 129(13)
Pages 2651-9
Published 2016-7-1
DOI 10.1242/jcs.189076
PII jcs.189076
PMID 27231092
PMC PMC4958304
MeSH Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism Animals Cadherins / metabolism Cell Membrane / genetics Cell Membrane / metabolism Cell Polarity / genetics Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Epithelium / growth & development Epithelium / metabolism Humans Microfilament Proteins / genetics* Microtubule-Associated Proteins / genetics* Microtubules / genetics Microvilli / genetics Microvilli / metabolism* Myosin Type V / genetics* Myosin Type V / metabolism Protein Transport / genetics rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism
IF 4.517
Times Cited 37
Drosophila DGRC#104055 DGRC#111536 DGRC#115285