論文 - 詳細
| RRC ID | 57352 |
|---|---|
| 著者 | Khanal I, Elbediwy A, Diaz de la Loza Mdel C, Fletcher GC, Thompson BJ. |
| タイトル | Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia. |
| ジャーナル | J Cell Sci |
| Abstract |
In epithelial tissues, polarisation of microtubules and actin microvilli occurs along the apical-basal axis of each cell, yet how these cytoskeletal polarisation events are coordinated remains unclear. Here, we examine the hierarchy of events during cytoskeletal polarisation in Drosophila melanogaster epithelia. Core apical-basal polarity determinants polarise the spectrin cytoskeleton to recruit the microtubule-binding proteins Patronin (CAMSAP1, CAMSAP2 and CAMSAP3 in humans) and Shortstop [Shot; MACF1 and BPAG1 (also known as DST) in humans] to the apical membrane domain. Patronin and Shot then act to polarise microtubules along the apical-basal axis to enable apical transport of Rab11 endosomes by the Nuf-Dynein microtubule motor complex. Finally, Rab11 endosomes are transferred to the MyoV (also known as Didum in Drosophila) actin motor to deliver the key microvillar determinant Cadherin 99C to the apical membrane to organise the biogenesis of actin microvilli. |
| 巻・号 | 129(13) |
| ページ | 2651-9 |
| 公開日 | 2016-7-1 |
| DOI | 10.1242/jcs.189076 |
| PII | jcs.189076 |
| PMID | 27231092 |
| PMC | PMC4958304 |
| MeSH | Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism Animals Cadherins / metabolism Cell Membrane / genetics Cell Membrane / metabolism Cell Polarity / genetics Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics Epithelium / growth & development Epithelium / metabolism Humans Microfilament Proteins / genetics* Microtubule-Associated Proteins / genetics* Microtubules / genetics Microvilli / genetics Microvilli / metabolism* Myosin Type V / genetics* Myosin Type V / metabolism Protein Transport / genetics rab GTP-Binding Proteins / genetics* rab GTP-Binding Proteins / metabolism |
| IF | 4.517 |
| 引用数 | 37 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104055 DGRC#111536 DGRC#115285 |