RRC ID 57831
著者 Lv X, He C, Huang C, Wang H, Hua G, Wang Z, Zhou J, Chen X, Ma B, Timm BK, Maclin V, Dong J, Rueda BR, Davis JS, Wang C.
タイトル Timely expression and activation of YAP1 in granulosa cells is essential for ovarian follicle development.
ジャーナル FASEB J
Abstract Although the role of the Hippo signaling pathway in development and tumorigenesis has been extensively studied in multiple organs, its role in ovarian follicle development remains largely unknown. Here, we report that Yes-Associated Protein 1 (YAP1), the major effector of Hippo signaling, is spatiotemporally expressed in ovarian granulosa cells and plays a critical role in the regulation of follicle development. We found that the active form of YAP1 (nuclear YAP1) was predominantly expressed in proliferative granulosa cells, whereas the inactive form of YAP1 (cytoplasmic YAP1) was mainly detected in luteal cells (terminally differentiated granulosa cells). Pharmacological inhibition of YAP1 activity disrupted mouse ovarian follicle development in vitro and in vivo. Foxl2 promoter-driven knockout of Yap1 in ovarian granulosa cells resulted in increased apoptosis of granulosa cells, decreased number of corpora lutea, reduced ovarian size, and subfertility in transgenic mice. However, Cyp19a1 promoter-driven knockout of Yap1 in differentiated granulosa cells of preovulatory follicles and luteal cells of corpora lutea had no effect on ovarian morphology and fertility. Mechanistic studies demonstrated that YAP1 interacted with epidermal growth factor receptor and TGF-β signaling pathways to regulate granulosa cell proliferation, differentiation, and survival. Results from this study identify YAP1 as a critical regulator of granulosa cell proliferation and differentiation. Balanced expression and activation of YAP1 is essential for follicle development and successful reproduction. YAP1 is a promising target for treatment of subfertility associated with abnormal granulosa cell function.-Lv, X., He, C., Huang, C., Wang, H., Hua, G., Wang, Z., Zhou, J., Chen, X., Ma, B., Timm, B. K., Maclin, V., Dong, J., Rueda, B. R., Davis, J. S., Wang, C. Timely expression and activation of YAP1 in granulosa cells is essential for ovarian follicle development.
巻・号 33(9)
ページ 10049-10064
公開日 2019-9-1
DOI 10.1096/fj.201900179RR
PMID 31199671
PMC PMC6704445
MeSH Adaptor Proteins, Signal Transducing / antagonists & inhibitors Adaptor Proteins, Signal Transducing / deficiency Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / physiology* Adult Animals Aromatase / genetics Cell Cycle Proteins / antagonists & inhibitors Cell Cycle Proteins / deficiency Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Cell Differentiation Cell Division Cell Line Cell Nucleus / metabolism Cytoplasm / metabolism ErbB Receptors / metabolism Female Forkhead Box Protein L2 / genetics Gene Knockout Techniques Genes, Synthetic Granulosa Cells / cytology Granulosa Cells / metabolism* Hippo Signaling Pathway Humans Mice Mice, Inbred C57BL Organ Culture Techniques Ovarian Follicle / cytology Ovarian Follicle / growth & development* Ovarian Follicle / metabolism Promoter Regions, Genetic Protein Serine-Threonine Kinases / physiology Protein Transport Recombinant Proteins / metabolism Signal Transduction / genetics Transforming Growth Factor beta / physiology Verteporfin / pharmacology YAP-Signaling Proteins
IF 4.966
引用数 4
リソース情報
ヒト・動物細胞 KGN(RCB1154)