論文 - 詳細
| RRC ID | 5790 |
|---|---|
| 著者 | Kumfer KT, Cook SJ, Squirrell JM, Eliceiri KW, Peel N, O'Connell KF, White JG. |
| タイトル | CGEF-1 and CHIN-1 regulate CDC-42 activity during asymmetric division in the Caenorhabditis elegans embryo. |
| ジャーナル | Mol Biol Cell |
| Abstract |
The anterior-posterior axis of the Caenorhabditis elegans embryo is elaborated at the one-cell stage by the polarization of the partitioning (PAR) proteins at the cell cortex. Polarization is established under the control of the Rho GTPase RHO-1 and is maintained by the Rho GTPase CDC-42. To understand more clearly the role of the Rho family GTPases in polarization and division of the early embryo, we constructed a fluorescent biosensor to determine the localization of CDC-42 activity in the living embryo. A genetic screen using this biosensor identified one positive (putative guanine nucleotide exchange factor [GEF]) and one negative (putative GTPase activating protein [GAP]) regulator of CDC-42 activity: CGEF-1 and CHIN-1. CGEF-1 was required for robust activation, whereas CHIN-1 restricted the spatial extent of CDC-42 activity. Genetic studies placed CHIN-1 in a novel regulatory loop, parallel to loop described previously, that maintains cortical PAR polarity. We found that polarized distributions of the nonmuscle myosin NMY-2 at the cell cortex are independently produced by the actions of RHO-1, and its effector kinase LET-502, during establishment phase and CDC-42, and its effector kinase MRCK-1, during maintenance phase. CHIN-1 restricted NMY-2 recruitment to the anterior during maintenance phase, consistent with its role in polarizing CDC-42 activity during this phase. |
| 巻・号 | 21(2) |
| ページ | 266-77 |
| 公開日 | 2010-1-15 |
| DOI | 10.1091/mbc.e09-01-0060 |
| PII | E09-01-0060 |
| PMID | 19923324 |
| PMC | PMC2808230 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Cell Division* Cell Polarity Embryo, Nonmammalian / cytology* Embryo, Nonmammalian / metabolism GTPase-Activating Proteins / chemistry GTPase-Activating Proteins / metabolism* Green Fluorescent Proteins / metabolism Guanine Nucleotide Exchange Factors / chemistry Guanine Nucleotide Exchange Factors / metabolism* Models, Biological Molecular Sequence Data Mutation / genetics Myosin Type II / metabolism Protein Binding Protein Transport Signal Transduction cdc42 GTP-Binding Protein / metabolism* rho GTP-Binding Proteins / metabolism |
| IF | 3.791 |
| 引用数 | 47 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1909 |