RRC ID |
5829
|
著者 |
Kuranaga E, Kanuka H, Tonoki A, Takemoto K, Tomioka T, Kobayashi M, Hayashi S, Miura M.
|
タイトル |
Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs.
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ジャーナル |
Cell
|
Abstract |
Caspase activation has been extensively studied in the context of apoptosis. However, caspases also control other cellular functions, although the mechanisms regulating caspases in nonapoptotic contexts remain obscure. Drosophila IAP1 (DIAP1) is an endogenous caspase inhibitor that is crucial for regulating cell death during development. Here we describe Drosophila IKK-related kinase (DmIKKvarepsilon) as a regulator of caspase activation in a nonapoptotic context. We show that DmIKKvarepsilon promotes degradation of DIAP1 through direct phosphorylation. Knockdown of DmIKKvarepsilon in the proneural clusters of the wing imaginal disc, in which nonapoptotic caspase activity is required for proper sensory organ precursor (SOP) development, stabilizes endogenous DIAP1 and affects Drosophila SOP development. Our results demonstrate that DmIKKvarepsilon is a determinant of DIAP1 protein levels and that it establishes the threshold of activity required for the execution of nonapoptotic caspase functions.
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巻・号 |
126(3)
|
ページ |
583-96
|
公開日 |
2006-8-11
|
DOI |
10.1016/j.cell.2006.05.048
|
PII |
S0092-8674(06)00909-3
|
PMID |
16887178
|
MeSH |
Animals
Apoptosis / genetics
Caspases / genetics
Caspases / metabolism*
Cells, Cultured
Down-Regulation / genetics
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / cytology
Drosophila melanogaster / embryology*
Drosophila melanogaster / metabolism
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology*
Embryo, Nonmammalian / metabolism
Embryonic Development / genetics
Gene Expression Regulation, Developmental / genetics
I-kappa B Kinase / genetics
I-kappa B Kinase / metabolism*
Inhibitor of Apoptosis Proteins / genetics
Inhibitor of Apoptosis Proteins / metabolism*
Phosphorylation
Sense Organs / abnormalities
Sense Organs / cytology
Sense Organs / metabolism
Wings, Animal / abnormalities
Wings, Animal / cytology
Wings, Animal / metabolism
|
IF |
38.637
|
引用数 |
101
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
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リソース情報 |
ショウジョウバエ |
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