RRC ID 58712
Author Borah A, Pillai SC, Rochani AK, Palaninathan V, Nakajima Y, Maekawa T, Kumar DS.
Title GANT61 and curcumin-loaded PLGA nanoparticles for GLI1 and PI3K/Akt-mediated inhibition in breast adenocarcinoma.
Journal Nanotechnology
Abstract Current conventional mono and combination therapeutic strategies often fail to target breast cancer tissue effectively due to tumor heterogeneity comprising cancer stem cells (CSCs) and bulk tumor cells. This is further associated with drug toxicity and resistivity in the long run. A nanomedicine platform incorporating combination anti-cancer treatment might overcome these challenges and generate synergistic anti-cancer effects and also reduce drug toxicity. GANT61 and curcumin were co-delivered via polymeric nanoparticles (NPs) for the first time to elicit enhanced anti-tumor activity against heterogeneous breast cancer cell line MCF-7. We adopted the single-emulsion-solvent evaporation method for the preparation of the therapeutic NPs. The GANT61-curcumin PLGA NPs were characterized for their size, shape and chemical properties, and anti-cancer cell studies were undertaken for the plausible explanation of our hypothesis. The synthesized GANT61-curcumin PLGA NPs had a spherical, smooth surface morphology, and an average size of 347.4 d. nm. The NPs induced cytotoxic effects in breast cancer cells at a mid-minimal dosage followed by cell death via autophagy and apoptosis, reduction in their target protein expression along with compromising the self-renewal property of CSCs as revealed by their in vitro cell studies. The dual-drug NPs thus provide a novel perspective on aiding existing anti-cancer nanomedicine therapies to target a heterogeneous tumor mass effectively.
Volume 31(18)
Pages 185102
Published 2020-5-1
DOI 10.1088/1361-6528/ab6d20
PMID 31952056
MeSH Adenocarcinoma / drug therapy Adenocarcinoma / pathology Adenocarcinoma / ultrastructure Animals Apoptosis / drug effects Autophagy / drug effects Breast Neoplasms / drug therapy* Breast Neoplasms / pathology Breast Neoplasms / ultrastructure Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects Curcumin / pharmacology Curcumin / therapeutic use* Drug Liberation Endocytosis / drug effects Female Humans MCF-7 Cells Mice Nanoparticles / chemistry* Nanoparticles / ultrastructure Particle Size Phosphatidylinositol 3-Kinases / metabolism* Photoelectron Spectroscopy Polylactic Acid-Polyglycolic Acid Copolymer / chemistry* Proto-Oncogene Proteins c-akt / metabolism* Pyridines / pharmacology Pyridines / therapeutic use* Pyrimidines / pharmacology Pyrimidines / therapeutic use* Spectroscopy, Fourier Transform Infrared Spheroids, Cellular / drug effects Spheroids, Cellular / pathology Static Electricity Zinc Finger Protein GLI1 / metabolism*
IF 3.551
Times Cited 2
Human and Animal Cells MCF7(RCB1904) L929