| RRC ID |
58712
|
| 著者 |
Borah A, Pillai SC, Rochani AK, Palaninathan V, Nakajima Y, Maekawa T, Kumar DS.
|
| タイトル |
GANT61 and curcumin-loaded PLGA nanoparticles for GLI1 and PI3K/Akt-mediated inhibition in breast adenocarcinoma.
|
| ジャーナル |
Nanotechnology
|
| Abstract |
Current conventional mono and combination therapeutic strategies often fail to target breast cancer tissue effectively due to tumor heterogeneity comprising cancer stem cells (CSCs) and bulk tumor cells. This is further associated with drug toxicity and resistivity in the long run. A nanomedicine platform incorporating combination anti-cancer treatment might overcome these challenges and generate synergistic anti-cancer effects and also reduce drug toxicity. GANT61 and curcumin were co-delivered via polymeric nanoparticles (NPs) for the first time to elicit enhanced anti-tumor activity against heterogeneous breast cancer cell line MCF-7. We adopted the single-emulsion-solvent evaporation method for the preparation of the therapeutic NPs. The GANT61-curcumin PLGA NPs were characterized for their size, shape and chemical properties, and anti-cancer cell studies were undertaken for the plausible explanation of our hypothesis. The synthesized GANT61-curcumin PLGA NPs had a spherical, smooth surface morphology, and an average size of 347.4 d. nm. The NPs induced cytotoxic effects in breast cancer cells at a mid-minimal dosage followed by cell death via autophagy and apoptosis, reduction in their target protein expression along with compromising the self-renewal property of CSCs as revealed by their in vitro cell studies. The dual-drug NPs thus provide a novel perspective on aiding existing anti-cancer nanomedicine therapies to target a heterogeneous tumor mass effectively.
|
| 巻・号 |
31(18)
|
| ページ |
185102
|
| 公開日 |
2020-5-1
|
| DOI |
10.1088/1361-6528/ab6d20
|
| PMID |
31952056
|
| MeSH |
Adenocarcinoma / drug therapy
Adenocarcinoma / pathology
Adenocarcinoma / ultrastructure
Animals
Apoptosis / drug effects
Autophagy / drug effects
Breast Neoplasms / drug therapy*
Breast Neoplasms / pathology
Breast Neoplasms / ultrastructure
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Curcumin / pharmacology
Curcumin / therapeutic use*
Drug Liberation
Endocytosis / drug effects
Female
Humans
MCF-7 Cells
Mice
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Particle Size
Phosphatidylinositol 3-Kinases / metabolism*
Photoelectron Spectroscopy
Polylactic Acid-Polyglycolic Acid Copolymer / chemistry*
Proto-Oncogene Proteins c-akt / metabolism*
Pyridines / pharmacology
Pyridines / therapeutic use*
Pyrimidines / pharmacology
Pyrimidines / therapeutic use*
Spectroscopy, Fourier Transform Infrared
Spheroids, Cellular / drug effects
Spheroids, Cellular / pathology
Static Electricity
Zinc Finger Protein GLI1 / metabolism*
|
| IF |
3.551
|
| 引用数 |
2
|
| リソース情報 |
| ヒト・動物細胞 |
MCF7(RCB1904)
L929 |
