論文 - 詳細
| RRC ID | 59359 |
|---|---|
| 著者 | Jenzer C, Simionato E, Largeau C, Scarcelli V, Lefebvre C, Legouis R. |
| タイトル | Autophagy mediates phosphatidylserine exposure and phagosome degradation during apoptosis through specific functions of GABARAP/LGG-1 and LC3/LGG-2. |
| ジャーナル | Autophagy |
| Abstract |
Phagocytosis and macroautophagy/autophagy are 2 processes involved in lysosome-mediated clearance of extracellular and intracellular components, respectively. Recent studies have identified the recruitment of the autophagic protein LC3 during phagocytosis of apoptotic corpses in what is now called LC3-associated phagocytosis (LAP). LAP is a distinct process from autophagy but it relies on some members of the autophagy pathway to allow efficient degradation of the phagocytosed cargo. We investigated whether both LC3/LGG-2 and GABARAP/LGG-1 are involved in phagocytosis of apoptotic corpses during embryonic development of Caenorhabditis elegans. We discovered that both LGG-1 and LGG-2 are involved in the correct elimination of apoptotic corpses, but that they have different functions. lgg-1 and lgg-2 mutants present a delay in phagocytosis of apoptotic cells but genetic analyses indicate that LGG-1 and LGG-2 act upstream and downstream of the engulfment pathways, respectively. Moreover, LGG-1 and LGG-2 display different cellular localizations with enrichment in apoptotic corpses and phagocytic cells, respectively. For both LGG-1 and LGG-2, subcellular localization is vesicular and dependent on UNC-51/ULK1, BEC-1/BECN1 and the lipidation machinery, indicating that their functions during phagocytosis of apoptotic corpses mainly rely on autophagy. Finally, we show that LGG-1 is involved in the exposure of the 'eat-me signal' phosphatidylserine at the surface of the apoptotic cell to allow its recognition by the phagocytic cell, whereas LGG-2 is involved in later steps of phagocytosis to allow efficient cell corpse clearance by mediating the maturation/degradation of the phagosome. |
| 巻・号 | 15(2) |
| ページ | 228-241 |
| 公開日 | 2019-2-1 |
| DOI | 10.1080/15548627.2018.1512452 |
| PMID | 30160610 |
| PMC | PMC6333449 |
| MeSH | Animals Apoptosis* Autophagy* Caenorhabditis elegans / cytology* Caenorhabditis elegans / embryology Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / metabolism* Lysosomes / metabolism Membrane Fusion Microtubule-Associated Proteins / metabolism* Models, Biological Phagocytosis Phagosomes / metabolism* Phagosomes / ultrastructure Phosphatidylserines / metabolism* |
| IF | 11.059 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3489 tm5755 tm2423 tm847 tm2253 |