論文 - 詳細
| RRC ID | 59571 |
|---|---|
| 著者 | Ouyang JPT, Folkmann A, Bernard L, Lee CY, Seroussi U, Charlesworth AG, Claycomb JM, Seydoux G. |
| タイトル | P Granules Protect RNA Interference Genes from Silencing by piRNAs. |
| ジャーナル | Dev Cell |
| Abstract |
P granules are perinuclear condensates in C. elegans germ cells proposed to serve as hubs for self/non-self RNA discrimination by Argonautes. We report that a mutant (meg-3 meg-4) that does not assemble P granules in primordial germ cells loses competence for RNA-interference over several generations and accumulates silencing small RNAs against hundreds of endogenous genes, including the RNA-interference genes rde-11 and sid-1. In wild type, rde-11 and sid-1 transcripts are heavily targeted by piRNAs and accumulate in P granules but maintain expression. In the primordial germ cells of meg-3 meg-4 mutants, rde-11 and sid-1 transcripts disperse in the cytoplasm with the small RNA biogenesis machinery, become hyper-targeted by secondary sRNAs, and are eventually silenced. Silencing requires the PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that maintains trans-generational silencing of piRNA targets. These observations support a "safe harbor" model for P granules in protecting germline transcripts from piRNA-initiated silencing. |
| 巻・号 | 50(6) |
| ページ | 716-728.e6 |
| 公開日 | 2019-9-23 |
| DOI | 10.1016/j.devcel.2019.07.026 |
| PII | S1534-5807(19)30632-X |
| PMID | 31402283 |
| PMC | PMC6764750 |
| MeSH | Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism Cell Nucleus / metabolism Cytoplasmic Granules / genetics* Embryo, Nonmammalian / metabolism Epigenesis, Genetic Genes, Helminth Genetic Loci Germ Cells / metabolism Models, Biological Mutation / genetics Phenotype RNA Interference* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Transcription, Genetic Up-Regulation / genetics |
| IF | 9.19 |
| 引用数 | 8 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1200 tm1019 tm892 |