| RRC ID |
59571
|
| 著者 |
Ouyang JPT, Folkmann A, Bernard L, Lee CY, Seroussi U, Charlesworth AG, Claycomb JM, Seydoux G.
|
| タイトル |
P Granules Protect RNA Interference Genes from Silencing by piRNAs.
|
| ジャーナル |
Dev Cell
|
| Abstract |
P granules are perinuclear condensates in C. elegans germ cells proposed to serve as hubs for self/non-self RNA discrimination by Argonautes. We report that a mutant (meg-3 meg-4) that does not assemble P granules in primordial germ cells loses competence for RNA-interference over several generations and accumulates silencing small RNAs against hundreds of endogenous genes, including the RNA-interference genes rde-11 and sid-1. In wild type, rde-11 and sid-1 transcripts are heavily targeted by piRNAs and accumulate in P granules but maintain expression. In the primordial germ cells of meg-3 meg-4 mutants, rde-11 and sid-1 transcripts disperse in the cytoplasm with the small RNA biogenesis machinery, become hyper-targeted by secondary sRNAs, and are eventually silenced. Silencing requires the PIWI-class Argonaute PRG-1 and the nuclear Argonaute HRDE-1 that maintains trans-generational silencing of piRNA targets. These observations support a "safe harbor" model for P granules in protecting germline transcripts from piRNA-initiated silencing.
|
| 巻・号 |
50(6)
|
| ページ |
716-728.e6
|
| 公開日 |
2019-9-23
|
| DOI |
10.1016/j.devcel.2019.07.026
|
| PII |
S1534-5807(19)30632-X
|
| PMID |
31402283
|
| PMC |
PMC6764750
|
| MeSH |
Animals
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / metabolism
Cell Nucleus / metabolism
Cytoplasmic Granules / genetics*
Embryo, Nonmammalian / metabolism
Epigenesis, Genetic
Genes, Helminth
Genetic Loci
Germ Cells / metabolism
Models, Biological
Mutation / genetics
Phenotype
RNA Interference*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Transcription, Genetic
Up-Regulation / genetics
|
| IF |
9.19
|
| 引用数 |
8
|
| リソース情報 |
| 線虫 |
tm1200
tm1019
tm892 |
