Reference - Detail
|Author||Matsumoto M, Sudo T, Maruyama M, Osada H, Tsujimoto M.|
|Title||Activation of p38 mitogen-activated protein kinase is crucial in osteoclastogenesis induced by tumor necrosis factor.|
Tumor necrosis factor (TNF) induces osteoclast differentiation from bone marrow cells in the presence of macrophage colony-stimulating factor. Treatment of bone marrow cells with SB203580 but not PD98059 inhibited TNF-induced osteoclast differentiation. In RAW264 cells which differentiate into osteoclast-like multinucleated cells by TNF treatment alone, activation of p38 mitogen-activated protein (MAP) kinase induced by murine TNF was comparable to and independent of the receptor activator of necrosis factor-kappaB ligand. Moreover, the number of multinucleated osteoclasts induced by TNF in bone marrow cell cultures derived from p38 MAP kinase gene deficient mice was significantly less than that from control mice. These results indicate that the p38 MAP kinase pathway plays a crucial role in TNF-mediated osteoclast differentiation.
|MeSH||Animals Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Bone Marrow Cells / enzymology Carrier Proteins / pharmacology Cell Differentiation / drug effects Cell Line Cell Nucleus / drug effects Cells, Cultured Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Flavonoids / pharmacology Giant Cells / cytology Giant Cells / drug effects Giant Cells / enzymology Imidazoles / pharmacology Intracellular Signaling Peptides and Proteins Macrophage Colony-Stimulating Factor / pharmacology Membrane Glycoproteins / pharmacology Mice Mice, Inbred C57BL Mice, Knockout Mitogen-Activated Protein Kinases / antagonists & inhibitors Mitogen-Activated Protein Kinases / deficiency Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Monocytes / cytology Monocytes / drug effects Monocytes / enzymology Osteoclasts / cytology* Osteoclasts / drug effects* Osteoclasts / enzymology Protein-Serine-Threonine Kinases / metabolism Pyridines / pharmacology RANK Ligand Receptor Activator of Nuclear Factor-kappa B Solubility Tumor Necrosis Factor-alpha / pharmacology* p38 Mitogen-Activated Protein Kinases|
|Human and Animal Cells||RAW 264(RCB0535)|