RRC ID 61490
Author Cao Z, Dou C, Li J, Tang X, Xiang J, Zhao C, Zhu L, Bai Y, Xiang Q, Dong S.
Title Cordycepin inhibits chondrocyte hypertrophy of mesenchymal stem cells through PI3K/Bapx1 and Notch signaling pathway.
Journal BMB Rep
Abstract Mesenchymal stem cells (MSCs) are widely used in cartilage tissue engineering to repair articular cartilage defects. However, hypertrophy of chondrocytes derived from MSCs might hinder the stabilization of hyaline cartilage. Thus, it is very important to find a suitable way to maintain the chondrogenic phenotype of chondrocytes. It has been reported that cordycepin has anti-inflammatory and anti-tumor functions. However, the role of cordycepin in chondrocyte hypertrophy remains unclear. Therefore, the objective of this study was to determine the effect of cordycepin on chondrogenesis and chondrocyte hypertrophy in MSCs and ATDC5 cells. Cordycepin upregulated chondrogenic markers including Sox9 and collagen type II while down-regulated hypertrophic markers including Runx2 and collagen type X. Further exploration showed that cordycepin promoted chondrogenesis through inhibiting Nrf2 while activating BMP signaling. Besides, cordycepin suppressed chondrocyte hypertrophy through PI3K/Bapx1 pathway and Notch signaling. Our results indicated cordycepin had the potential to maintain chondrocyte phenotype and reconstruct engineered cartilage. [BMB Reports 2016; 49(10): 548-553].
Volume 49(10)
Pages 548-553
Published 2016-10-1
DOI 10.5483/bmbrep.2016.49.10.071
PII 3543
PMID 27439604
PMC PMC5227296
MeSH Antineoplastic Agents / chemistry Antineoplastic Agents / pharmacology* Cell Differentiation / drug effects* Cells, Cultured Chondrocytes / cytology Chondrocytes / drug effects Chondrocytes / metabolism Chondrogenesis / drug effects Collagen Type II / metabolism Collagen Type X / metabolism Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Deoxyadenosines / chemistry Deoxyadenosines / pharmacology* Down-Regulation / drug effects Homeodomain Proteins / metabolism Humans Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / drug effects Mesenchymal Stem Cells / metabolism NF-E2-Related Factor 2 / genetics NF-E2-Related Factor 2 / metabolism Phosphatidylinositol 3-Kinases / metabolism Real-Time Polymerase Chain Reaction Receptors, Notch / genetics Receptors, Notch / metabolism SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism Signal Transduction / drug effects* Transcription Factors / metabolism Up-Regulation / drug effects
IF 3.167
Human and Animal Cells ATDC5(RCB0565)