論文 - 詳細
| RRC ID | 61525 |
|---|---|
| 著者 | Akiyama H, Furukawa S, Wakisaka S, Maeda T. |
| タイトル | Elevated expression of CTRP3/cartducin contributes to promotion of osteosarcoma cell proliferation. |
| ジャーナル | Oncol Rep |
| Abstract |
CTRP3/cartducin, a novel secretory protein, is a member of the C1q and tumor necrosis factor (TNF)-related protein (CTRP) superfamily, and plays important roles in regulating both embryonic cartilage development and postnatal longitudinal bone growth. CTRP3/cartducin is expressed in human osteosarcomas. We hypothesized that CTRP3/cartducin might have a role in osteosarcoma tumor growth and metastasis. Murine osteosarcoma cell lines, NHOS and LM8, were used as a model. RT-PCR analysis showed that the mRNA level of CTRP3/cartducin was increased in these two murine osteosarcoma cell lines compared with its level in normal murine osteoblast MC3T3-E1 cells. Western blot analysis showed that the protein level of CTRP3/cartducin was also increased in these two osteosarcoma cell lines. Stimulation of NHOS and LM8 cells by CTRP3/cartducin promoted tumor cell growth but not migration in vitro. Further, CTRP3/cartducin stimulation led to the activation of extracellular signal-regulated kinase 1/2 (ERK1/2) in these two osteosarcoma cell lines. MAPK/ERK kinase 1/2 (MEK1/2) inhibitor, U0126, blocked CTRP3/cartducin-induced cell proliferation. These results suggest that CTRP3/cartducin expression may play a role in osteosarcoma tumor growth associated with activation of the ERK1/2 signaling pathway. |
| 巻・号 | 21(6) |
| ページ | 1477-81 |
| 公開日 | 2009-6-1 |
| DOI | 10.3892/or_00000377 |
| PMID | 19424626 |
| MeSH | Adipokines Animals Bone Neoplasms / genetics Bone Neoplasms / metabolism* Bone Neoplasms / pathology Cell Line, Tumor Cell Movement Cell Proliferation* / drug effects Gene Expression Regulation, Neoplastic Mice Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors Mitogen-Activated Protein Kinase 3 / metabolism Osteosarcoma / genetics Osteosarcoma / metabolism* Osteosarcoma / pathology Phosphorylation Protein Kinase Inhibitors / pharmacology Proteins / genetics Proteins / metabolism* RNA, Messenger / metabolism Recombinant Proteins / metabolism Signal Transduction Time Factors Up-Regulation |
| IF | 3.417 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |