| RRC ID |
61917
|
| 著者 |
Song IW, Li WR, Chen LY, Shen LF, Liu KM, Yen JJ, Chen YJ, Chen YJ, Kraus VB, Wu JY, Lee MT, Chen YT.
|
| タイトル |
Palmitoyl acyltransferase, Zdhhc13, facilitates bone mass acquisition by regulating postnatal epiphyseal development and endochondral ossification: a mouse model.
|
| ジャーナル |
PLoS One
|
| Abstract |
ZDHHC13 is a member of DHHC-containing palmitoyl acyltransferases (PATs) family of enzymes. It functions by post-translationally adding 16-carbon palmitate to proteins through a thioester linkage. We have previously shown that mice carrying a recessive Zdhhc13 nonsense mutation causing a Zdhcc13 deficiency develop alopecia, amyloidosis and osteoporosis. Our goal was to investigate the pathogenic mechanism of osteoporosis in the context of this mutation in mice. Body size, skeletal structure and trabecular bone were similar in Zdhhc13 WT and mutant mice at birth. Growth retardation and delayed secondary ossification center formation were first observed at day 10 and at 4 weeks of age, disorganization in growth plate structure and osteoporosis became evident in mutant mice. Serial microCT from 4-20 week-olds revealed that Zdhhc13 mutant mice had reduced bone mineral density. Through co-immunoprecipitation and acyl-biotin exchange, MT1-MMP was identified as a direct substrate of ZDHHC13. In cells, reduction of MT1-MMP palmitoylation affected its subcellular distribution and was associated with decreased VEGF and osteocalcin expression in chondrocytes and osteoblasts. In Zdhhc13 mutant mice epiphysis where MT1-MMP was under palmitoylated, VEGF in hypertrophic chondrocytes and osteocalcin at the cartilage-bone interface were reduced based on immunohistochemical analyses. Our results suggest that Zdhhc13 is a novel regulator of postnatal skeletal development and bone mass acquisition. To our knowledge, these are the first data to suggest that ZDHHC13-mediated MT1-MMP palmitoylation is a key modulator of bone homeostasis. These data may provide novel insights into the role of palmitoylation in the pathogenesis of human osteoporosis.
|
| 巻・号 |
9(3)
|
| ページ |
e92194
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0092194
|
| PII |
PONE-D-13-43117
|
| PMID |
24637783
|
| PMC |
PMC3956893
|
| MeSH |
Acyltransferases / deficiency
Acyltransferases / genetics
Acyltransferases / metabolism*
Animals
Animals, Newborn
Bone Density
Cartilage / pathology*
Cartilage / physiopathology*
Cell Proliferation
Chondrocytes / metabolism
Chondrocytes / pathology
Epiphyses / blood supply
Epiphyses / growth & development*
Epiphyses / pathology*
Epiphyses / physiopathology
Gene Expression Regulation, Developmental
Growth Plate / pathology
HEK293 Cells
Humans
Hypertrophy
Lipoylation
Matrix Metalloproteinase 14 / metabolism
Mice
Models, Animal
Mutation / genetics
Organ Size
Osteoblasts / metabolism
Osteocalcin / metabolism
Osteogenesis*
Osteoporosis / diagnostic imaging
Osteoporosis / pathology
Osteoporosis / physiopathology
Protein Binding
Radiography
Subcellular Fractions / enzymology
Vascular Endothelial Growth Factor A / metabolism
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
ATDC5(RCB0565) |
