| RRC ID |
62758
|
| 著者 |
Huang X, Liu C, Hao C, Tang Q, Liu R, Lin S, Zhang L, Yan W.
|
| タイトル |
Identification of altered microRNAs and mRNAs in the cumulus cells of PCOS patients: miRNA-509-3p promotes oestradiol secretion by targeting MAP3K8.
|
| ジャーナル |
Reproduction
|
| Abstract |
Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women and is characterised by polycystic ovaries, hyperandrogenism and chronic anovulation. Although the clinical and biochemical signs of PCOS are typically heterogeneous, abnormal folliculogenesis is considered a common characteristic of PCOS. Our aim is to identify the altered miRNA and mRNA expression profiles in the cumulus cells of PCOS patients to investigate their molecular function in the aetiology and pathophysiology of PCOS. In this study, the miRNA expression profiles of the cumulus cell samples isolated from five PCOS and five control patients were determined by an miRNA microarray. At the same time, the altered mRNA profiles of the same cumulus cell samples were also identified by a cDNA microarray. From the microarray data, 17 miRNAs and 1263 mRNAs showed significantly different expression in the PCOS cumulus cells. The differentially expressed miRNA-509-3p and its potential target gene (MAP3K8) were identified from the miRNA and mRNA microarrays respectively. The expression of miRNA-509-3p was up-regulated and MAP3K8 was down-regulated in the PCOS cumulus cells. The direct interaction between miRNA-509-3p and MAP3K8 was confirmed by a luciferase activity assay in KGN cells. In addition, miRNA-509-3p mimics or inhibitor transfection tests in KGN cells further confirmed that miRNA-509-3p improved oestradiol (E2) secretion by inhibiting the expression of MAP3K8 These results help to characterise the pathogenesis of anovulation in PCOS, especially the regulation of E2 production.
|
| 巻・号 |
151(6)
|
| ページ |
643-55
|
| 公開日 |
2016-6-1
|
| DOI |
10.1530/REP-16-0071
|
| PII |
REP-16-0071
|
| PMID |
27001999
|
| MeSH |
Adult
Case-Control Studies
Cell Communication
Cells, Cultured
Cumulus Cells / metabolism*
Cumulus Cells / pathology
Estradiol / metabolism*
Female
Gene Expression Profiling
Humans
MAP Kinase Kinase Kinases / genetics
MAP Kinase Kinase Kinases / metabolism*
MicroRNAs / genetics*
Oligonucleotide Array Sequence Analysis
Polycystic Ovary Syndrome / genetics*
Polycystic Ovary Syndrome / pathology
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism*
RNA, Messenger / genetics*
|
| IF |
3.206
|
| リソース情報 |
| ヒト・動物細胞 |
KGN(RCB1154) |
