RRC ID 6335
著者 Yamamoto M, Morita R, Mizoguchi T, Matsuo H, Isoda M, Ishitani T, Chitnis AB, Matsumoto K, Crump JG, Hozumi K, Yonemura S, Kawakami K, Itoh M.
タイトル Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions.
ジャーナル Development
Abstract In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.
巻・号 137(15)
ページ 2527-37
公開日 2010-8-1
DOI 10.1242/dev.051011
PII dev.051011
PMID 20573700
PMC PMC2927672
MeSH 3T3 Cells Animals Body Patterning* COS Cells Calcium-Binding Proteins / metabolism* Cell Lineage* Chlorocebus aethiops Endocytosis Gene Expression Regulation, Developmental* Intercellular Signaling Peptides and Proteins / metabolism* Jagged-1 Protein Membrane Proteins / metabolism* Mice Models, Biological Notochord / physiology* Receptors, Notch / metabolism* Serrate-Jagged Proteins Two-Hybrid System Techniques Ubiquitin / metabolism Ubiquitin-Protein Ligases / metabolism* Zebrafish Zebrafish Proteins / metabolism*
IF 5.611
引用数 54
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ゼブラフィッシュ SAGFF(LF)214A Tg(UAS:GFP)