RRC ID 63452
Author Hayashida Y, Ikeda Y, Sawada K, Kawai K, Kato T, Kakehi Y, Araki N.
Title Invention of a novel photodynamic therapy for tumors using a photosensitizing PI3K inhibitor.
Journal Int J Cancer
Abstract XL147 (SAR245408, pilaralisib), an ATP-competitive pan-class I phosphoinositide 3-kinase (PI3K) inhibitor, is a promising new anticancer drug. We examined the effect of the PI3K inhibitor on PC3 prostate cancer cells under a fluorescence microscope and found that XL147-treated cancer cells are rapidly injured by blue wavelength (430 nm) light irradiation. During the irradiation, the cancer cells treated with 0.2-2 μM XL147 showed cell surface blebbing and cytoplasmic vacuolation and died within 15 min. The extent of cell injury/death was dependent on the dose of XL147 and the light power of the irradiation. These findings suggest that XL147 might act as a photosensitizing reagent in photodynamic therapy (PDT) for cancer. Moreover, the cytotoxic effect of photosensitized XL147 was reduced by pretreatment with other ATP-competitive PI3K inhibitors such as LY294002, suggesting that the cytotoxic effect of photosensitized XL147 is facilitated by binding to PI3K in cells. In a single-cell illumination analysis using a fluorescent probe to identify reactive oxygen species (ROS), significantly increased ROS production was observed in the XL147-treated cells when the cell was illuminated with blue light. Taken together, it is conceivable that XL147, which is preferentially accumulated in cancer cells, could be photosensitized by blue light to produce ROS to kill cancer cells. This study will open up new possibilities for PDT using anticancer drugs.
Volume 139(3)
Pages 700-11
Published 2016-8-1
DOI 10.1002/ijc.30097
PMID 26989815
MeSH Animals Antineoplastic Agents / pharmacology* Apoptosis / drug effects Apoptosis / radiation effects Cell Line, Tumor Chromones / pharmacology Dose-Response Relationship, Drug Humans Light* Macrophages / drug effects Macrophages / metabolism Macrophages / radiation effects Mice Molecular Imaging Morpholines / pharmacology Neoplasms / metabolism* Neoplasms / therapy* Phosphatidylinositol 3-Kinases / metabolism* Phosphoinositide-3 Kinase Inhibitors* Photochemotherapy* / methods Photosensitizing Agents / therapeutic use* Quinoxalines / pharmacology Reactive Oxygen Species / metabolism Sulfonamides / pharmacology
IF 5.145
Human and Animal Cells A431(RCB0202) RAW 264(RCB0535)