| RRC ID |
6381
|
| 著者 |
Joo HJ, Yim YH, Jeong PY, Jin YX, Lee JE, Kim H, Jeong SK, Chitwood DJ, Paik YK.
|
| タイトル |
Caenorhabditis elegans utilizes dauer pheromone biosynthesis to dispose of toxic peroxisomal fatty acids for cellular homoeostasis.
|
| ジャーナル |
Biochem J
|
| Abstract |
Caenorhabditis elegans excretes a dauer pheromone or daumone composed of ascarylose and a fatty acid side chain, the perception of which enables worms to enter the dauer state for long-term survival in an adverse environment. During the course of elucidation of the daumone biosynthetic pathway in which DHS-28 and DAF-22 are involved in peroxisomal beta-oxidation of VLCFAs (very long-chain fatty acids), we sought to investigate the physiological consequences of a deficiency in daumone biosynthesis in C. elegans. Our results revealed that two mutants, dhs-28(tm2581) and daf-22(ok693), lacked daumones and thus were dauer defective; this coincided with massive accumulation of fatty acyl-CoAs (up to 100-fold) inside worm bodies compared with levels in wild-type N2 worms. Furthermore, the deficiency in daumone biosynthesis and the massive accumulation of fatty acids and their acyl-CoAs caused severe developmental defects with reduced life spans (up to 30%), suggesting that daumone biosynthesis is be an essential part of C. elegans homoeostasis, affecting survival and maintenance of optimal physiological conditions by metabolizing some of the toxic non-permissible peroxisomal VLCFAs from the worm body in the form of readily excretable daumones.
|
| 巻・号 |
422(1)
|
| ページ |
61-71
|
| 公開日 |
2009-7-29
|
| DOI |
10.1042/BJ20090513
|
| PII |
BJ20090513
|
| PMID |
19496754
|
| MeSH |
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / metabolism
Cytoplasmic Granules / metabolism
Embryonic Development
Fatty Acids / biosynthesis*
Gene Expression Regulation, Developmental
Genes, Helminth
Hexoses / biosynthesis
Homeostasis*
Longevity
Models, Biological
Mutation / genetics
Oxidation-Reduction
Peroxisomes / metabolism*
Phenotype
Pheromones / biosynthesis*
|
| IF |
4.097
|
| 引用数 |
55
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2581 |
