RRC ID 6381
著者 Joo HJ, Yim YH, Jeong PY, Jin YX, Lee JE, Kim H, Jeong SK, Chitwood DJ, Paik YK.
タイトル Caenorhabditis elegans utilizes dauer pheromone biosynthesis to dispose of toxic peroxisomal fatty acids for cellular homoeostasis.
ジャーナル Biochem J
Abstract Caenorhabditis elegans excretes a dauer pheromone or daumone composed of ascarylose and a fatty acid side chain, the perception of which enables worms to enter the dauer state for long-term survival in an adverse environment. During the course of elucidation of the daumone biosynthetic pathway in which DHS-28 and DAF-22 are involved in peroxisomal beta-oxidation of VLCFAs (very long-chain fatty acids), we sought to investigate the physiological consequences of a deficiency in daumone biosynthesis in C. elegans. Our results revealed that two mutants, dhs-28(tm2581) and daf-22(ok693), lacked daumones and thus were dauer defective; this coincided with massive accumulation of fatty acyl-CoAs (up to 100-fold) inside worm bodies compared with levels in wild-type N2 worms. Furthermore, the deficiency in daumone biosynthesis and the massive accumulation of fatty acids and their acyl-CoAs caused severe developmental defects with reduced life spans (up to 30%), suggesting that daumone biosynthesis is be an essential part of C. elegans homoeostasis, affecting survival and maintenance of optimal physiological conditions by metabolizing some of the toxic non-permissible peroxisomal VLCFAs from the worm body in the form of readily excretable daumones.
巻・号 422(1)
ページ 61-71
公開日 2009-7-29
DOI 10.1042/BJ20090513
PII BJ20090513
PMID 19496754
MeSH Animals Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Cytoplasmic Granules / metabolism Embryonic Development Fatty Acids / biosynthesis* Gene Expression Regulation, Developmental Genes, Helminth Hexoses / biosynthesis Homeostasis* Longevity Models, Biological Mutation / genetics Oxidation-Reduction Peroxisomes / metabolism* Phenotype Pheromones / biosynthesis*
IF 4.097
引用数 55
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm2581