論文 - 詳細
| RRC ID | 63920 |
|---|---|
| 著者 | Matsuoka S, Sato K, Maruki-Imamura R, Noutoshi Y, Okabe T, Kojima H, Umezawa T. |
| タイトル | Identification of novel compounds that inhibit SnRK2 kinase activity by high-throughput screening. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Abscisic acid (ABA) is a major phytohormone that regulates abiotic stress responses and development.
SNF1-rerated protein kinase 2 (SnRK2) is a key regulator of ABA signaling. To isolate compounds which
directly affect SnRK2 activity, we optimized a fluorescence-based system for high-throughput screening
(HTS) of SnRK2 kinase regulators. Using this system, we screened a chemical library consisting of 16,000
compounds and identified ten compounds (INH1-10) as potential SnRK2 inhibitors. Further character ization of these compounds by in vitro phosphorylation assays confirmed that three of the ten com pounds were SnRK2-specific kinase inhibitors. In contrast, seven of ten compounds inhibited ABA responsive gene expression in Arabidopsis cells. From these results, INH1 was identified as a SnRK2-
specific inhibitor in vitro and in vivo. We propose that INH1 could be a lead compound of chemical
tools for studying ABA responses in various plant species. |
| 巻・号 | 537 |
| ページ | 57-63 |
| 公開日 | 2020-12-29 |
| DOI | 10.1016/j.bbrc.2020.12.046 |
| PII | S0006-291X(20)32223-3 |
| PMID | 33385806 |
| MeSH | Abscisic Acid / pharmacology Arabidopsis / drug effects Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / antagonists & inhibitors* Arabidopsis Proteins / metabolism Gene Expression Regulation, Plant / drug effects Genes, Plant High-Throughput Screening Assays* Phosphorylation / drug effects Protein Kinase Inhibitors / analysis* Protein Kinase Inhibitors / chemistry Protein Kinase Inhibitors / pharmacology* Protein Serine-Threonine Kinases / antagonists & inhibitors* Protein Serine-Threonine Kinases / metabolism Recombinant Proteins / metabolism |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |