RRC ID 6394
Author Luo J, Shah S, Riabowol K, Mains PE.
Title The Caenorhabditis elegans ing-3 gene regulates ionizing radiation-induced germ-cell apoptosis in a p53-associated pathway.
Journal Genetics
Abstract The inhibitor of growth (ING) family of type II tumor suppressors are encoded by five genes in mammals and by three genes in Caenorhabditis elegans. All ING proteins contain a highly conserved plant homeodomain (PHD) zinc finger. ING proteins are activated by stresses, including ionizing radiation, leading to the activation of p53. ING proteins in mammals and yeast have recently been shown to read the histone code in a methylation-sensitive manner to regulate gene expression. Here we identify and characterize ing-3, the C. elegans gene with the highest sequence identity to the human ING3 gene. ING-3 colocalizes with chromatin in embryos, the germline, and somatic cells. The ing-3 gene is part of an operon but is also transcribed from its own promoter. Both ing-3(RNAi) and ing-3 mutant strains demonstrate that the gene likely functions in concert with the C. elegans p53 homolog, cep-1, to induce germ-cell apoptosis in response to ionizing radiation. Somatically, the ing-3 mutant has a weak kinker uncoordinated (kinker Unc) phenotype, indicating a possible neuronal function.
Volume 181(2)
Pages 473-82
Published 2009-2
DOI 10.1534/genetics.107.080515
PII genetics.107.080515
PMID 19015549
PMC PMC2644941
MeSH Animals Animals, Genetically Modified Apoptosis / genetics* Apoptosis / radiation effects Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans / radiation effects Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism DNA Primers / genetics DNA, Helminth / genetics Gene Expression Genes, Helminth* Genes, Tumor Suppressor* Germ Cells / cytology Germ Cells / radiation effects Homeodomain Proteins / genetics Humans Immunohistochemistry Mutation RNA Interference Species Specificity Tissue Distribution Trans-Activators / genetics Tumor Suppressor Protein p53 / genetics Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism
IF 4.075
Times Cited 16
C.elegans tm2169 tm2530 tm2830 tm2180