論文 - 詳細
| RRC ID | 64100 |
|---|---|
| 著者 | Hu Y, Galkin AV, Wu C, Reddy V, Su AI. |
| タイトル | CAFET algorithm reveals Wnt/PCP signature in lung squamous cell carcinoma. |
| ジャーナル | PLoS One |
| Abstract |
We analyzed the gene expression patterns of 138 Non-Small Cell Lung Cancer (NSCLC) samples and developed a new algorithm called Coverage Analysis with Fisher's Exact Test (CAFET) to identify molecular pathways that are differentially activated in squamous cell carcinoma (SCC) and adenocarcinoma (AC) subtypes. Analysis of the lung cancer samples demonstrated hierarchical clustering according to the histological subtype and revealed a strong enrichment for the Wnt signaling pathway components in the cluster consisting predominantly of SCC samples. The specific gene expression pattern observed correlated with enhanced activation of the Wnt Planar Cell Polarity (PCP) pathway and inhibition of the canonical Wnt signaling branch. Further real time RT-PCR follow-up with additional primary tumor samples and lung cancer cell lines confirmed enrichment of Wnt/PCP pathway associated genes in the SCC subtype. Dysregulation of the canonical Wnt pathway, characterized by increased levels of β-catenin and epigenetic silencing of negative regulators, has been reported in adenocarcinoma of the lung. Our results suggest that SCC and AC utilize different branches of the Wnt pathway during oncogenesis. |
| 巻・号 | 6(10) |
| ページ | e25807 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0025807 |
| PII | PONE-D-11-05185 |
| PMID | 22016777 |
| PMC | PMC3189939 |
| MeSH | Adenocarcinoma / genetics Adenocarcinoma / pathology Algorithms* Carcinoma, Non-Small-Cell Lung / genetics Carcinoma, Non-Small-Cell Lung / pathology Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / pathology* Cell Polarity / genetics* Cell Survival / genetics Cluster Analysis Computational Biology / methods* Humans Lung Neoplasms / genetics Lung Neoplasms / pathology* Signal Transduction / genetics Transcriptome* Wnt Proteins / genetics* Wnt Proteins / metabolism |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-14(RCB0446) |