Reference - Detail
| RRC ID | 64110 |
|---|---|
| Author | Kishimoto KN, Itoi E. |
| Title | Lithium chloride enhances cathepsin H expression and BMP-4 degradation in C3H10T1/2 cells. |
| Journal | Biomed Res Int |
| Abstract |
The effect of canonical Wnt/β-catenin signaling on chondrogenic differentiation induced by transfection of BMP4 expressing plasmid was analyzed. Lithium chloride (LiCl) which mimics canonical Wnt/β-catenin signaling was added to cells transfected with BMP4 expressing plasmid. Although BMP4 mRNA expression was not affected by LiCl, LiCl decreased BMP4 protein accumulation. Gene expression analysis exhibited upregulation of cathepsin H by LiCl treatment. Gene silencing of cathepsin H enhanced BMP4 protein accumulation from BMP4 expressing cells. These results suggested that cathepsin H is regulated by Wnt/β-catenin signaling and plays an important role in the regulation of BMP4 biological activity. |
| Volume | 2013 |
| Pages | 143742 |
| Published | 2013-1-1 |
| DOI | 10.1155/2013/143742 |
| PMID | 24312905 |
| PMC | PMC3842059 |
| MeSH | Animals Bone Morphogenetic Protein 4 / genetics Bone Morphogenetic Protein 4 / metabolism* Cathepsin H / metabolism* Cell Aggregation / drug effects Cell Aggregation / genetics Cell Differentiation / drug effects Cell Differentiation / genetics Chondrogenesis / drug effects Chondrogenesis / genetics Electroporation Gene Expression Profiling Gene Expression Regulation / drug effects Gene Silencing / drug effects Humans Lithium Chloride / pharmacology* Mice Plasmids / metabolism Proteolysis / drug effects* Signal Transduction / drug effects Signal Transduction / genetics Staining and Labeling TCF Transcription Factors / metabolism Transfection beta Catenin / metabolism |
| IF | 2.276 |
| Resource | |
| Human and Animal Cells | 10T1/2(RCB0247) |