RRC ID 650
Author Tanno K, Takeda K.
Title On the origin of six-rowed barley with brittle rachis, agriocrithon [Hordeum vulgare ssp. vulgare f. agriocrithon (Aberg) Bowd.], based on a DNA marker closely linked to the vrs1 (six-row gene) locus.
Journal Theor. Appl. Genet.
Abstract The origin of six-rowed cultivated barley has been revealed to be more complex since the discovery of agriocrithon, a six-rowed barley with brittle rachis. The present study investigates whether such six-rowed brittle barley is wild or hybrid in nature, by analyzing genetic diversity at the cMWG699 marker locus, which is closely linked to the vrs1 (six-row gene) locus. DNA sequence analysis for 42 accessions showed only three types in six-rowed brittle barleys; in contrast, nine sequence types were found in ten wild barleys, ssp. spontaneum, in our previous study. Nucleotide diversities for the six-rowed brittle barley were 2.8-4.5 times lower than that for the ssp. spontaneum at this marker locus. The three sequence types found in the six-rowed brittle barley also appeared in the six-rowed cultivated barley. A cross-allelism test confirmed that the six-rowed character of the six-rowed brittle barley was controlled by the vrs1 locus. The nucleotide diversity and genealogy demonstrated that f. agriocrithon does not have the same level of diversity as found in wild barley, ssp. spontaneum. Consequently, f. agriocrithon does not appear to represent genuinely wild populations, but more probably originated from hybridization between ssp. spontaneum and six-rowed cultivated barley.
Volume 110(1)
Pages 145-50
Published 2004-12
DOI 10.1007/s00122-004-1816-4
PMID 15526085
MeSH Alleles Base Sequence Crosses, Genetic DNA, Plant / genetics Genes, Plant* Genetic Linkage Genetic Markers Hordeum / anatomy & histology* Hordeum / classification Hordeum / genetics* Phenotype Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide Sequence Homology, Nucleic Acid
IF 3.93
Times Cited 12
WOS Category AGRONOMY HORTICULTURE PLANT SCIENCES GENETICS & HEREDITY
Resource
Barley OUH641 OUH662 OUH663 OUH765 OUH768 OUH785 OUH786 OUH787 OUH788 OUH789 etc.