Reference - Detail
| RRC ID | 65160 |
|---|---|
| Author | Osakabe K, Wada N, Murakami E, Miyashita N, Osakabe Y. |
| Title | Genome editing in mammalian cells using the CRISPR type I-D nuclease. |
| Journal | Nucleic Acids Res |
| Abstract |
Adoption of CRISPR-Cas systems, such as CRISPR-Cas9 and CRISPR-Cas12a, has revolutionized genome engineering in recent years; however, application of genome editing with CRISPR type I-the most abundant CRISPR system in bacteria-remains less developed. Type I systems, such as type I-E, and I-F, comprise the CRISPR-associated complex for antiviral defense ('Cascade': Cas5, Cas6, Cas7, Cas8 and the small subunit) and Cas3, which degrades the target DNA; in contrast, for the sub-type CRISPR-Cas type I-D, which lacks a typical Cas3 nuclease in its CRISPR locus, the mechanism of target DNA degradation remains unknown. Here, we found that Cas10d is a functional nuclease in the type I-D system, performing the role played by Cas3 in other CRISPR-Cas type I systems. The type I-D system can be used for targeted mutagenesis of genomic DNA in human cells, directing both bi-directional long-range deletions and short insertions/deletions. Our findings suggest the CRISPR-Cas type I-D system as a unique effector pathway in CRISPR that can be repurposed for genome engineering in eukaryotic cells. |
| Volume | 49(11) |
| Pages | 6347-6363 |
| Published | 2021-6-21 |
| DOI | 10.1093/nar/gkab348 |
| PII | 6291161 |
| PMID | 34076237 |
| PMC | PMC8216271 |
| MeSH | CRISPR-Associated Proteins / chemistry CRISPR-Associated Proteins / genetics CRISPR-Associated Proteins / metabolism* CRISPR-Cas Systems* Endodeoxyribonucleases / chemistry Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Gene Editing* HEK293 Cells Humans Mutagenesis Mutation |
| IF | 11.502 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |