RRC ID 65354
Author Lister-Shimauchi EH, Dinh M, Maddox P, Ahmed S.
Title Gametes deficient for Pot1 telomere binding proteins alter levels of telomeric foci for multiple generations.
Journal Commun Biol
Abstract Deficiency for telomerase results in transgenerational shortening of telomeres. However, telomeres have no known role in transgenerational epigenetic inheritance. C. elegans Protection Of Telomeres 1 (Pot1) proteins form foci at the telomeres of germ cells that disappear at fertilization and gradually accumulate during development. We find that gametes from mutants deficient for Pot1 proteins alter levels of telomeric foci for multiple generations. Gametes from pot-2 mutants give rise to progeny with abundant POT-1::mCherry and mNeonGreen::POT-2 foci throughout development, which persists for six generations. In contrast, gametes from pot-1 mutants or pot-1; pot-2 double mutants induce diminished Pot1 foci for several generations. Deficiency for MET-2, SET-25, or SET-32 methyltransferases, which promote heterochromatin formation, results in gametes that induce diminished Pot1 foci for several generations. We propose that C. elegans POT-1 may interact with H3K9 methyltransferases during pot-2 mutant gametogenesis to induce a persistent form of transgenerational epigenetic inheritance that causes constitutively high levels of heterochromatic Pot1 foci.
Volume 4(1)
Pages 158
Published 2021-2-4
DOI 10.1038/s42003-020-01624-7
PII 10.1038/s42003-020-01624-7
PMID 33542458
PMC PMC7862594
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism DNA-Binding Proteins / deficiency* DNA-Binding Proteins / genetics Epigenesis, Genetic Female Gametogenesis* Gene Expression Regulation, Developmental Germ Cells / metabolism* Heredity Histone Methyltransferases / genetics Histone Methyltransferases / metabolism Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / metabolism Male Telomere / genetics Telomere / metabolism* Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism*
C.elegans tm1400 tm1620 tm1200