RRC ID 65665
Author Aguilar Lucero D, Cantoia A, Sánchez-López C, Binolfi A, Mogk A, Ceccarelli EA, Rosano GL.
Title Structural features of the plant N-recognin ClpS1 and sequence determinants in its targets that govern substrate selection.
Journal FEBS Lett
Abstract In the N-degron pathway of protein degradation of Escherichia coli, the N-recognin ClpS identifies substrates bearing N-terminal phenylalanine, tyrosine, tryptophan, or leucine and delivers them to the caseinolytic protease (Clp). Chloroplasts contain the Clp system, but whether chloroplastic ClpS1 adheres to the same constraints is unknown. Moreover, the structural underpinnings of substrate recognition are not completely defined. We show that ClpS1 recognizes canonical residues of the E. coli N-degron pathway. The residue in second position influences recognition (especially in N-terminal ends starting with leucine). N-terminal acetylation abrogates recognition. ClpF, a ClpS1-interacting partner, does not alter its specificity. Substrate binding provokes local remodeling of residues in the substrate-binding cavity of ClpS1. Our work strongly supports the existence of a chloroplastic N-degron pathway.
Volume 595(11)
Pages 1525-1541
Published 2021-6-1
DOI 10.1002/1873-3468.14081
PMID 33792910
MeSH Adaptor Proteins, Signal Transducing / chemistry* Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / chemistry* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Binding Sites Carrier Proteins / chemistry* Carrier Proteins / genetics Carrier Proteins / metabolism Chloroplasts / enzymology* Chloroplasts / genetics Cloning, Molecular Conserved Sequence Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Gene Expression Genetic Vectors / chemistry Genetic Vectors / metabolism Leucine / chemistry Leucine / metabolism Models, Molecular Phenylalanine / chemistry Phenylalanine / metabolism Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Protein Interaction Domains and Motifs Proteolysis Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Substrate Specificity Tryptophan / chemistry Tryptophan / metabolism Tyrosine / chemistry Tyrosine / metabolism
IF 3.057
Arabidopsis / Cultured plant cells, genes pda14908