論文 - 詳細
| RRC ID | 66583 |
|---|---|
| 著者 | Lee JE, Yoon SH, Lee GY, Lee DH, Huh CS, Kim GB. |
| タイトル | Chryseobacterium vaccae sp. nov., isolated from raw cow's milk. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Strain CA7T, a Gram-stain-negative, non-motile, non-spore-forming, aerobic and rod-shaped bacterial strain, was isolated from raw cow's milk collected from a farm affiliated with Chung-Ang University, Anseong, Korea, and characterized by a polyphasic approach. Optimal growth of strain CA7T was observed on tryptic soy agar at 30 °C and pH 7.0 with 0 % NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CA7T belonged to the genus Chryseobacterium. The most closely related strains (16S rRNA gene sequence similarity indicated in parentheses), based on the phylogenetic analysis, were Chryseobacterium rhizosphaerae KCTC 22548T (98.08 %), Chryseobacterium nakagawai CCUG 60563T (98.61 %), Chryseobacterium jejuense KACC 12501T (97.85 %) and Chryseobacterium aurantiacum KCTC 62135T (97.78 %). Whole genome sequencing indicated that the genome size was 5 125 723 bp and had a DNA G+C content of 37.4 mol%. Average nucleotide identity values for strain CA7T with C. rhizosphaerae, C. nakagawai, C. jejuense, C. aurantiacum, and the type species of the genus Chryseobacterium, C. gleum, were 80.2, 79.8, 79.8, 79.6 and 80.4 %, respectively. The digital DNA-DNA hybridization values of CA7T compared to C. rhizosphaerae, C. nakagawai, C. jejuense, C. aurantiacum and C. gleum were 24.1, 23.9, 23.9, 23.7 and 24.3 %, respectively. The major fatty acids were iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl), iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone-6 was the only respiratory quinone. The major polar lipid was phosphatidylethanolamine. Based on this polyphasic taxonomic study, strain CA7T represents a novel species of the genus Chryseobacterium for which the name Chryseobacterium vaccae sp. nov. is proposed. The type strain is CA7T (=KACC 21402T=JCM 33749T). |
| 巻・号 | 70(9) |
| ページ | 4859-4866 |
| 公開日 | 2020-9-1 |
| DOI | 10.1099/ijsem.0.004250 |
| PMID | 32515727 |
| MeSH | Animals Bacterial Typing Techniques Base Composition Cattle Chryseobacterium / classification* Chryseobacterium / isolation & purification DNA, Bacterial / genetics Fatty Acids / chemistry Milk / microbiology* Nucleic Acid Hybridization Phosphatidylethanolamines / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Republic of Korea Sequence Analysis, DNA Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM33749 JCM2410 |