RRC ID 6727
著者 Muramoto T, Urushihara H.
タイトル Small GTPase RacF2 affects sexual cell fusion and asexual development in Dictyostelium discoideum through the regulation of cell adhesion.
ジャーナル Dev Growth Differ
Abstract Cells of Dictyostelium discoideum become sexually mature when submerged and in darkness, and fuse with opposite mating-type cells as gametes. The gene for a Rho GTPase, RacF2, is one of the extremely gamete-enriched genes (>100-fold) identified by us previously. Here, we isolated knockout, overexpression, constitutively active and dominant negative mutants of RacF2, and analyzed their phenotypes. These mutants showed anomalies in the extent of sexual cell fusion and asexual development as well as in EDTA-sensitive cell-cell adhesion. It is suggested that RacF2 controls the process of sexual and asexual development through the regulation of cellular adhesiveness. An analysis of the expression of all 18 rac family genes by real-time polymerase chain reaction revealed that four additional genes, rac1b, rac1c, racF1 and racG, were induced during maturation, suggesting their possible involvement in sexual cell interactions.
巻・号 48(3)
ページ 199-208
公開日 2006-4-1
DOI 10.1111/j.1440-169X.2006.00857.x
PII DGD
PMID 16573737
MeSH Animals Cell Adhesion / physiology Cells, Cultured Dictyostelium / genetics* Dictyostelium / growth & development* Dictyostelium / physiology Fertilization / physiology* Gene Expression Regulation, Developmental Mutation Phenotype Reproduction, Asexual / physiology* Time Factors rac GTP-Binding Proteins / genetics rho GTP-Binding Proteins / genetics* rho GTP-Binding Proteins / physiology*
IF 1.723
引用数 8
WOS 分野 CELL BIOLOGY DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 G01272 S00257 S00296 S00297 S00298 S90099 S90100 S90101 S90102 S90103 S90104 S90105 S90106 S90107 S90108 S90109 S90110 S90111 S90112 S90113 S90114 S90115 S90116 S90117 S90261 S90262 S90263