論文 - 詳細
| RRC ID | 68025 |
|---|---|
| 著者 | Fukuda T, Sugita S, Inatome R, Yanagi S. |
| タイトル | CAMDI, a novel disrupted in schizophrenia 1 (DISC1)-binding protein, is required for radial migration. |
| ジャーナル | J Biol Chem |
| Abstract |
Centrosomes play a crucial role in the directed migration of developing neurons. However, the underlying mechanism is poorly understood. This study has identified a novel disrupted in schizophrenia 1 (DISC1)-interacting protein, named CAMDI after coiled-coil protein associated with myosin II and DISC1, which translocates to the centrosome in a DISC1-dependent manner. Knockdown of CAMDI by shRNA revealed severely impaired radial migration with disoriented centrosomes. A yeast two-hybrid screen identified myosin II as a binding protein of CAMDI. CAMDI interacts preferentially with phosphomyosin II and induces an accumulation of phosphomyosin II at the centrosome in a DISC1-dependent manner. Interestingly, one single nucleotide polymorphism of the CAMDI gene (R828W) is identified, and its gene product was found to reduce the binding ability to phosphomyosin II. Furthermore, mice with overexpression of R828W in neurons exhibit an impaired radial migration. Our findings indicate that CAMDI is required for radial migration probably through DISC1 and myosin II-mediated centrosome positioning during neuronal development. |
| 巻・号 | 285(52) |
| ページ | 40554-61 |
| 公開日 | 2010-12-24 |
| DOI | 10.1074/jbc.M110.179481 |
| PII | S0021-9258(19)76222-X |
| PMID | 20956536 |
| PMC | PMC3003354 |
| MeSH | Animals Base Sequence COS Cells Cell Movement / physiology* Centrosome / metabolism* Chlorocebus aethiops Gene Knockdown Techniques HEK293 Cells Humans Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism* Mice Molecular Sequence Data Myosin Type II / genetics Myosin Type II / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neurogenesis / physiology* Neurons / cytology Neurons / metabolism* |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |