論文 - 詳細
| RRC ID | 68069 |
|---|---|
| 著者 | Chen L, Zong R, Zhou J, Ge L, Zhou T, Ma JX, Liu Z, Zhou Y. |
| タイトル | The oxidant role of 4-hydroxynonenal in corneal epithelium. |
| ジャーナル | Sci Rep |
| Abstract |
4-Hydroxynonenal (4-HNE or HNE) is a main endogenous product of cellular lipid peroxidation in tissues and is reported to play pathogenic roles in eye diseases. Here we investigated the association between 4-HNE and oxidative stress in the corneal epithelium. 4-HNE suppressed the cell viability of human corneal epithelial cells (HCE) in a concentration dependent manner. 4-HNE significantly increased the level of 3-Nitrotyrosine (3-NT), a marker of oxidative stress, in HCE cells and corneal epithelium of rats by immunofluorescent staining and Western blot analysis. To its underlying mechanistic on ROS system, 4-HNE elevated the ROS generation enzyme NADPH oxidase 4 (NOX4) and induced the activation of NF-E2-related factor-2 (NRF2) and its downstream effectors: NAD(P)H dehydrogenase (quinone 1) (NQO1) and glutathione S-transferase P (GSTP). Furthermore, N-acetylcysteine (NAC), an antioxidant and ROS scavenger, antagonized the inhibitory and oxidant effects of 4-HNE on the corneal epithelial cells. In conclusion, 4-HNE plays an oxidant role in the corneal epithelium and this work provides a new strategy for the pathogenesis and treatment of corneal diseases. |
| 巻・号 | 5 |
| ページ | 10630 |
| 公開日 | 2015-5-29 |
| DOI | 10.1038/srep10630 |
| PII | srep10630 |
| PMID | 26023743 |
| PMC | PMC4448499 |
| MeSH | Acetylcysteine / pharmacology Aldehydes / metabolism* Aldehydes / pharmacology Animals Cells, Cultured Drug Antagonism Epithelial Cells / drug effects Epithelial Cells / metabolism Epithelium, Corneal / drug effects Epithelium, Corneal / metabolism* Humans Male NADPH Oxidase 4 NADPH Oxidases / metabolism NF-E2-Related Factor 2 / metabolism Oxidants / metabolism* Oxidants / pharmacology Oxidative Stress / drug effects Rats Reactive Oxygen Species / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |