RRC ID 68069
Author Chen L, Zong R, Zhou J, Ge L, Zhou T, Ma JX, Liu Z, Zhou Y.
Title The oxidant role of 4-hydroxynonenal in corneal epithelium.
Journal Sci Rep
Abstract 4-Hydroxynonenal (4-HNE or HNE) is a main endogenous product of cellular lipid peroxidation in tissues and is reported to play pathogenic roles in eye diseases. Here we investigated the association between 4-HNE and oxidative stress in the corneal epithelium. 4-HNE suppressed the cell viability of human corneal epithelial cells (HCE) in a concentration dependent manner. 4-HNE significantly increased the level of 3-Nitrotyrosine (3-NT), a marker of oxidative stress, in HCE cells and corneal epithelium of rats by immunofluorescent staining and Western blot analysis. To its underlying mechanistic on ROS system, 4-HNE elevated the ROS generation enzyme NADPH oxidase 4 (NOX4) and induced the activation of NF-E2-related factor-2 (NRF2) and its downstream effectors: NAD(P)H dehydrogenase (quinone 1) (NQO1) and glutathione S-transferase P (GSTP). Furthermore, N-acetylcysteine (NAC), an antioxidant and ROS scavenger, antagonized the inhibitory and oxidant effects of 4-HNE on the corneal epithelial cells. In conclusion, 4-HNE plays an oxidant role in the corneal epithelium and this work provides a new strategy for the pathogenesis and treatment of corneal diseases.
Volume 5
Pages 10630
Published 2015-5-29
DOI 10.1038/srep10630
PII srep10630
PMID 26023743
PMC PMC4448499
MeSH Acetylcysteine / pharmacology Aldehydes / metabolism* Aldehydes / pharmacology Animals Cells, Cultured Drug Antagonism Epithelial Cells / drug effects Epithelial Cells / metabolism Epithelium, Corneal / drug effects Epithelium, Corneal / metabolism* Humans Male NADPH Oxidase 4 NADPH Oxidases / metabolism NF-E2-Related Factor 2 / metabolism Oxidants / metabolism* Oxidants / pharmacology Oxidative Stress / drug effects Rats Reactive Oxygen Species / metabolism
IF 3.998
Human and Animal Cells HCE-T(RCB2280)