| RRC ID |
68069
|
| Author |
Chen L, Zong R, Zhou J, Ge L, Zhou T, Ma JX, Liu Z, Zhou Y.
|
| Title |
The oxidant role of 4-hydroxynonenal in corneal epithelium.
|
| Journal |
Sci Rep
|
| Abstract |
4-Hydroxynonenal (4-HNE or HNE) is a main endogenous product of cellular lipid peroxidation in tissues and is reported to play pathogenic roles in eye diseases. Here we investigated the association between 4-HNE and oxidative stress in the corneal epithelium. 4-HNE suppressed the cell viability of human corneal epithelial cells (HCE) in a concentration dependent manner. 4-HNE significantly increased the level of 3-Nitrotyrosine (3-NT), a marker of oxidative stress, in HCE cells and corneal epithelium of rats by immunofluorescent staining and Western blot analysis. To its underlying mechanistic on ROS system, 4-HNE elevated the ROS generation enzyme NADPH oxidase 4 (NOX4) and induced the activation of NF-E2-related factor-2 (NRF2) and its downstream effectors: NAD(P)H dehydrogenase (quinone 1) (NQO1) and glutathione S-transferase P (GSTP). Furthermore, N-acetylcysteine (NAC), an antioxidant and ROS scavenger, antagonized the inhibitory and oxidant effects of 4-HNE on the corneal epithelial cells. In conclusion, 4-HNE plays an oxidant role in the corneal epithelium and this work provides a new strategy for the pathogenesis and treatment of corneal diseases.
|
| Volume |
5
|
| Pages |
10630
|
| Published |
2015-5-29
|
| DOI |
10.1038/srep10630
|
| PII |
srep10630
|
| PMID |
26023743
|
| PMC |
PMC4448499
|
| MeSH |
Acetylcysteine / pharmacology
Aldehydes / metabolism*
Aldehydes / pharmacology
Animals
Cells, Cultured
Drug Antagonism
Epithelial Cells / drug effects
Epithelial Cells / metabolism
Epithelium, Corneal / drug effects
Epithelium, Corneal / metabolism*
Humans
Male
NADPH Oxidase 4
NADPH Oxidases / metabolism
NF-E2-Related Factor 2 / metabolism
Oxidants / metabolism*
Oxidants / pharmacology
Oxidative Stress / drug effects
Rats
Reactive Oxygen Species / metabolism
|
| IF |
3.998
|
| Resource |
| Human and Animal Cells |
HCE-T(RCB2280) |
