| RRC ID |
68504
|
| 著者 |
Gunduz M, Ouchida M, Fukushima K, Ito S, Jitsumori Y, Nakashima T, Nagai N, Nishizaki K, Shimizu K.
|
| タイトル |
Allelic loss and reduced expression of the ING3, a candidate tumor suppressor gene at 7q31, in human head and neck cancers.
|
| ジャーナル |
Oncogene
|
| Abstract |
Loss of heterozygosity (LOH) has been frequently detected at chromosome 7q31 region in human head and neck squamous cell carcinomas (HNSCC) and many other cancers, suggesting the existence of tumor suppressor genes (TSG). We analysed LOH at 7q31 region in 49 HNSCC by using six polymorphic microsatellite markers and found allelic deletion in 48% (22/46) of the informative cases. We detected two preferentially deleted regions, one is around D7S643 and the other around D7S486. When we redefined the map of 7q31 region according to the contiguous sequences, a recently identified gene, ING3, was found in the proximity of D7S643. ING3 protein harbors the PHD domain highly homologous among ING family proteins, in which we previously found mutations in a related gene, ING1. As only one missense mutation of the ING3 gene was found in HNSCC, we examined the expression level. Reverse-transcription-PCR analysis demonstrated decreased or no expression of ING3 mRNA in 50% of primary tumors as compared with that of matched normal samples. Especially, about 63% of tongue and larynx tumors showed the decrease and a tendency of higher mortality was observed in cases with decreased ING3 expression. All these findings suggest a possibility that the ING3 gene functions as a TSG in a subset of HNSCC.
|
| 巻・号 |
21(28)
|
| ページ |
4462-70
|
| 公開日 |
2002-6-27
|
| DOI |
10.1038/sj.onc.1205540
|
| PMID |
12080476
|
| MeSH |
Amino Acid Sequence
Blotting, Northern
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / metabolism
Carcinoma, Squamous Cell / pathology
Chromosome Deletion
Chromosome Mapping
Chromosomes, Human, Pair 7 / genetics*
DNA Primers / chemistry
DNA, Neoplasm / metabolism
Genes, Tumor Suppressor*
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / metabolism
Head and Neck Neoplasms / pathology
Homeodomain Proteins
Humans
Loss of Heterozygosity
Luciferases / metabolism
Microsatellite Repeats / genetics
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Promoter Regions, Genetic
Proteins / genetics*
Proteins / metabolism
RNA / metabolism
RNA, Neoplasm / metabolism
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Trans-Activators
Tumor Suppressor Proteins
|
| IF |
7.971
|
| リソース情報 |
| ヒト・動物細胞 |
SCCKN(RCB0441)
SCCTF(RCB0442)
T3M-1
HSQ-89(RCB0789) |
