RRC ID 69502
Author Takabatake K, Konishi H, Arita T, Kataoka S, Shibamoto J, Furuke H, Takaki W, Shoda K, Shimizu H, Yamamoto Y, Komatsu S, Shiozaki A, Fujiwara H, Okamoto K, Otsuji E.
Title Anterior gradient 2 regulates cancer progression in TP53‑wild‑type esophageal squamous cell carcinoma.
Journal Oncol Rep
Abstract Anterior gradient 2 (AGR2) reportedly promotes tumor growth and has an unfavorable impact on survival in several cancers. However, no comprehensive functional analysis of AGR2 in esophageal squamous cell carcinoma (ESCC) has been performed. In the present study, the function and clinical significance of AGR2 were examined using ESCC cell lines and clinical samples. AGR2 was upregulated in EC tissue and ESCC cell lines. The downregulation of AGR2 suppressed cell proliferation and increased the proportion of G2/M‑phase cells and phosphorylation of p53 in TP53‑wild‑type ESCC and osteosarcoma cells. However, these changes were not observed in TP53‑mutant ESCC cells. In addition, immunohistochemistry results demonstrated that high AGR2 and low p53 expression levels in ESCC tissues were correlated with a worse prognosis. These results suggested that although AGR2 enhanced cell proliferation by inhibiting p53 phosphorylation in TP53‑wild‑type ESCC, the same mechanism did not regulate cell functions in TP53‑mutant ESCC. Thus, AGR2 served an important role in ESCC progression and might be a useful prognostic marker in patients with TP53‑wild‑type ESCC.
Volume 46(6)
Published 2021-12-1
DOI 10.3892/or.2021.8211
PII 260
PMID 34713298
MeSH Bone Neoplasms / genetics Bone Neoplasms / pathology Cell Line, Tumor Disease Progression* Down-Regulation Esophageal Neoplasms / genetics* Esophageal Neoplasms / pathology Esophageal Squamous Cell Carcinoma / genetics* Esophageal Squamous Cell Carcinoma / pathology Gene Expression Regulation, Neoplastic / genetics Humans Mucoproteins / genetics* Mucoproteins / metabolism Oncogene Proteins / genetics* Oncogene Proteins / metabolism Osteosarcoma / genetics Osteosarcoma / pathology Phosphorylation Tumor Suppressor Protein p53 / metabolism Up-Regulation
IF 3.417
Human and Animal Cells Saos-2(RCB0428)