論文 - 詳細
| RRC ID | 70181 |
|---|---|
| 著者 | Osugi T, Miyasaka N, Shiraishi A, Matsubara S, Satake H. |
| タイトル | Cionin, a vertebrate cholecystokinin/gastrin homolog, induces ovulation in the ascidian Ciona intestinalis type A. |
| ジャーナル | Sci Rep |
| Abstract |
Cionin is a homolog of vertebrate cholecystokinin/gastrin that has been identified in the ascidian Ciona intestinalis type A. The phylogenetic position of ascidians as the closest living relatives of vertebrates suggests that cionin can provide clues to the evolution of endocrine/neuroendocrine systems throughout chordates. Here, we show the biological role of cionin in the regulation of ovulation. In situ hybridization demonstrated that the mRNA of the cionin receptor, Cior2, was expressed specifically in the inner follicular cells of pre-ovulatory follicles in the Ciona ovary. Cionin was found to significantly stimulate ovulation after 24-h incubation. Transcriptome and subsequent Real-time PCR analyses confirmed that the expression levels of receptor tyrosine kinase (RTK) signaling genes and a matrix metalloproteinase (MMP) gene were significantly elevated in the cionin-treated follicles. Of particular interest is that an RTK inhibitor and MMP inhibitor markedly suppressed the stimulatory effect of cionin on ovulation. Furthermore, inhibition of RTK signaling reduced the MMP gene expression in the cionin-treated follicles. These results provide evidence that cionin induces ovulation by stimulating MMP gene expression via the RTK signaling pathway. This is the first report on the endogenous roles of cionin and the induction of ovulation by cholecystokinin/gastrin family peptides in an organism. |
| 巻・号 | 11(1) |
| ページ | 10911 |
| 公開日 | 2021-5-25 |
| DOI | 10.1038/s41598-021-90295-3 |
| PII | 10.1038/s41598-021-90295-3 |
| PMID | 34035343 |
| PMC | PMC8149874 |
| MeSH | Animals Ciona intestinalis / genetics Ciona intestinalis / physiology* Female Gene Expression Profiling In Situ Hybridization Matrix Metalloproteinases / genetics Matrix Metalloproteinases / metabolism Neuropeptides / metabolism* Neuropeptides / pharmacology Ovary / drug effects Ovary / metabolism* Ovulation Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism Sequence Analysis, RNA Signal Transduction / drug effects |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |