RRC ID 70181
Author Osugi T, Miyasaka N, Shiraishi A, Matsubara S, Satake H.
Title Cionin, a vertebrate cholecystokinin/gastrin homolog, induces ovulation in the ascidian Ciona intestinalis type A.
Journal Sci Rep
Abstract Cionin is a homolog of vertebrate cholecystokinin/gastrin that has been identified in the ascidian Ciona intestinalis type A. The phylogenetic position of ascidians as the closest living relatives of vertebrates suggests that cionin can provide clues to the evolution of endocrine/neuroendocrine systems throughout chordates. Here, we show the biological role of cionin in the regulation of ovulation. In situ hybridization demonstrated that the mRNA of the cionin receptor, Cior2, was expressed specifically in the inner follicular cells of pre-ovulatory follicles in the Ciona ovary. Cionin was found to significantly stimulate ovulation after 24-h incubation. Transcriptome and subsequent Real-time PCR analyses confirmed that the expression levels of receptor tyrosine kinase (RTK) signaling genes and a matrix metalloproteinase (MMP) gene were significantly elevated in the cionin-treated follicles. Of particular interest is that an RTK inhibitor and MMP inhibitor markedly suppressed the stimulatory effect of cionin on ovulation. Furthermore, inhibition of RTK signaling reduced the MMP gene expression in the cionin-treated follicles. These results provide evidence that cionin induces ovulation by stimulating MMP gene expression via the RTK signaling pathway. This is the first report on the endogenous roles of cionin and the induction of ovulation by cholecystokinin/gastrin family peptides in an organism.
Volume 11(1)
Pages 10911
Published 2021-5-25
DOI 10.1038/s41598-021-90295-3
PII 10.1038/s41598-021-90295-3
PMID 34035343
PMC PMC8149874
MeSH Animals Ciona intestinalis / genetics Ciona intestinalis / physiology* Female Gene Expression Profiling In Situ Hybridization Matrix Metalloproteinases / genetics Matrix Metalloproteinases / metabolism Neuropeptides / metabolism* Neuropeptides / pharmacology Ovary / drug effects Ovary / metabolism* Ovulation Receptor Protein-Tyrosine Kinases / genetics Receptor Protein-Tyrosine Kinases / metabolism Sequence Analysis, RNA Signal Transduction / drug effects
IF 3.998
C.intestinalis / (O.japonicus) Wild C. int